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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Expression

Mammalian

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Article

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA AKT1

Plasmid

#78778

Purpose

To overexpress AKT1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

AKT1 (AKT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pATT-Dest

Plasmid

#79770

Purpose

Staging plasmid for use in DIP transposition

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Synthetic Biology; Staging plasmid for transposit…

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6HA

Plasmid

#99520

Purpose

C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xHA

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-BTK

Plasmid

#23918

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BTK (BTK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMTEZ-Kir2.1

Plasmid

#32641

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Kir2.1 (Kcnj2 Mouse)

Expression

Bacterial

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSK

Plasmid

#23941

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSK (CSK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SRPK1

Plasmid

#23582

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SRPK1 (SRPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52379

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4

Expression

Mammalian

Promoter

OCT4

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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GST TopBP1 (aa 32-1522) His

Plasmid

#20375

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

Insert

TopBP1 (TOPBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Starts at aa32

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pAHYB-GFP

Plasmid

#85074

Purpose

Expression of GFP in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al Yeast. 2014 Apr;31(4):115-25.

Insert

GFP

Expression

Yeast

Promoter

AOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_G3BP1

Plasmid

#127106

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mEosEM-Lamin A

Plasmid

#132707

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

lamin A

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) WT V5 His

Plasmid

#119287

Purpose

CIP2A WT V5 overexpression

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Tags

V5 His

Expression

Mammalian

Promoter

T7

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only