159,557 results

1. pTF102

   Plasmid

   #134774

   Purpose

   A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

   Depositor

   Kan Wang

   Article

   Frame et al Plant Physiol. 2002 May;129(1):13-22. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV/hSyn-SCLM

   Plasmid

   #216760

   Purpose

   AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   hSyn (human synapsin I)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30a(+)-PE2

   Plasmid

   #180015

   Purpose

   Expressing PE2 protein in E.coli for protein purification

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Lac operator-controlled T7 promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1ab-Prkd1

   Plasmid

   #139314

   Purpose

   Diacylglycerol biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T208A, N313D

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

   Plasmid

   #216533

   Purpose

   An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

   Depositor

   Yuki Kambe

   Article

   Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

   Insert

   axon-jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   GAP43

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcdna3.1 hygro mCherry

   Plasmid

   #128057

   Purpose

   Mammalian expression vector with N terminal mCherry

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hHS1

   Plasmid

   #159169

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCFD5_w

   Plasmid

   #112645

   Purpose

   Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

   Depositor

   Michael Boutros

   Insert

   U6:3-tRNA-sgRNA expression cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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