Addgene

We narrowed to 6,924 results for: ef1a;

Showing: 1921 - 1940 of 6924 results

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pGH389

Plasmid

#230847

Purpose

Ef1a-AID*-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

AID*-dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH385

Plasmid

#230848

Purpose

Ef1a-dCas9-AID*-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

dCas9-AID*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH398

Plasmid

#230854

Purpose

Ef1a-rAPOBEC1-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

rAPOBEC1-n'Cas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH905

Plasmid

#230844

Purpose

Ef1a-PmCDA1-BE3-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

PmCDA1-BE3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH483

Plasmid

#230835

Purpose

Ef1a-dCas9-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH401

Plasmid

#230852

Purpose

Ef1a-rAPOBEC1-nCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

rAPOBEC1-nCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH484

Plasmid

#230838

Purpose

Ef1a-AID*-dCas9-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

AID*-dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH903

Plasmid

#230857

Purpose

Ef1a-PmCDA1-BE3-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

PmCDA1-BE3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH880

Plasmid

#230839

Purpose

Ef1a-dCas9-AID*-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

dCas9-AID*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJD1

Plasmid

#222374

Purpose

Recombinant human IgG1 antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF125

Plasmid

#222373

Purpose

Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG (partial)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKER-Clover

Plasmid

#85974

Purpose

Expresses Clover from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

Clover

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDD426

Plasmid

#202079

Purpose

Frame +1/-0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-N144

Plasmid

#66810

Purpose

2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

Reverse tetracycline-controlled transactivator

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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