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Addgene

We narrowed to 6,508 results for: human c myc

Showing: 1921 - 1940 of 6508 results
  1. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid
    #171995
    Purpose
    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Insert
    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Insert
    Dark MitoTrap
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. HIS_TEV_hsSYX3

    Plasmid
    #179290
    Purpose
    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site
    Depositor
    Insert
    Syx
    Use
    Tags
    His6 + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7lac
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. 8522-E10

    Plasmid
    #234295
    Purpose
    Gateway ORF Entry clone of human SMAD2 with stop codon (for native or N-terminal fusions); S464A/S465A/S467A mutations (C-terminal phosphorylation sites dead)
    Depositor
    Insert
    SMAD2 S464A/S465A/S467A (SMAD2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S464A/S465A/S467A; C-terminal phosphorylation sit…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pDONR221-WT-long-UBASH3A-Ctag

    Plasmid
    #192097
    Purpose
    Human wild-type long-form UBASH3A in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. R777-E174 Hs.PIK3R2-nostop

    Plasmid
    #70458
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. R777-E172 Hs.PIK3R1-nostop

    Plasmid
    #70456
    Purpose
    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    PIK3R1 (PIK3R1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. R777-E190 Hs.PREX2-nostop

    Plasmid
    #70474
    Purpose
    Gateway ORF clone of human PREX2 [NM_024870.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    PREX2 (PREX2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1921 - 1940 of 6508 results