We narrowed to 4,195 results for: spl
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Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
ExpressionMammalianMutation5' and 3' splice sites mutationsAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB3
Plasmid#63104PurposeExpression of HSPB3 (small HSP) tagged with V5 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthspb3 (HSPB3 Human)
TagsV5ExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGK111
Plasmid#228126PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SFFV-NR4A2-Brd 336
Plasmid#219265PurposeTranscription factor NR4A2 with a specific barcode assigned.DepositorAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
SFFV-CEBPB-Brd 226
Plasmid#219169PurposeTranscription factor CEBPB with a specific barcode assigned.DepositorAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-TAOK3
Plasmid#172993Purposeconstitutive expression of FLAG-tagged TAOK3DepositorAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantPromoter'long' CaMV35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1411 GFP-TDP43 FYW-S
Plasmid#118800PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to S in CTD of full-length TDP43Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1412 GFP-TDP43 FYW-L
Plasmid#118801PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to L in CTD of full-length TDP43Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUB2" (SNRPB2 Human)
TagsfSNAPAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNa-delta S
Plasmid#107983Purposebacterial expression of full-length OPNDepositorAvailable sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-NSarQ27G-VMAn-FKBP
Plasmid#70229PurposeExpresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
Tags3XFLAG, FK506- and rapamycin-binding protein (FKB…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Wnt2B2 Non-STOP
Plasmid#35889DepositorAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
TagsEGFP and mCherryExpressionMammalianAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIGR1-Cdc42-2
Plasmid#179518PurposeRetroviral expression of Cdc42 splice variant 2DepositorAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP-26sFur-B2AD-tevs-KKMP
Plasmid#179644PurposeAND gate (TEVP/HCVP) SEAP RELEASE constructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEAP RELEASE
ExpressionMammalianAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-535)
Plasmid#135436PurposeExpresses SFPQ 276-535 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
TagsHexahistidineExpressionBacterialPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry
Plasmid#133322PurposeBacterial expression of TDP43 with FYW-S mutant CTDDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionBacterialMutationAll FYW in the TDP43 IDRs mutated to SAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_hE2F7mg_PP7-tag
Plasmid#73078Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-endDepositorInsertE2F7 (E2F7 Human)
Tags5'-PP7-tag RNAExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by intronsAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only