Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,972 results for: try

Clear keywords
Showing: 1921 - 1940 of 2972 results

  1. pUAS-kkv-mos::NG

    Plasmid
    #138958
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4
    Depositor
    Paul Adler
    Publication
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    8 bp that are in the region that encodes the cata…
    Promoter
    UAS
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    138958-plasmid-map-sequence-id-274779

    Enlarge

  2. mILK-L207W-EFGP

    Plasmid
    #176900
    Purpose
    mouse ILK with L207W point mutation cloned into pEGFP-N1
    Depositor
    Sara Wickström
    Publication
    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Integrin-linked kinase (Ilk Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Leucine 207 to Tryptophane
    Promoter
    CNV
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    176900-plasmid-map-sequence-id-357256

    Enlarge

  4. pN1-mCherry-NKD2

    Plasmid
    #176641
    Purpose
    Expresses NKD2-mCherry fusion protein in mammalian cells
    Depositor
    Sonal Srikanth
    Publication
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Tags
    mCherry tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176641-plasmid-map-sequence-id-349875

    Enlarge

  7. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , T1785P)-HA

    Plasmid
    #239773
    Purpose
    Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C, T1785P
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239773-plasmid-map-sequence-id-477101

    Enlarge

  11. Epac-S-H170

    Plasmid
    #170352
    Purpose
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170352-plasmid-map-sequence-id-345817

    Enlarge

  12. p3x-Flag Gli3 37R3-2

    Plasmid
    #120305
    Purpose
    Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C
    Depositor
    David Liu
    Publication
    Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Intein 37R3-2, Gli3
    Use
    Synthetic Biology
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Contains mutations as reported in 2011 Chemistry …
    Promoter
    CMV
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120305-plasmid-map-sequence-id-234346

    Enlarge

  14. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Publication
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

    Enlarge

  18. FGFR2 V564F 3xFlag

    Plasmid
    #110108
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Valine 564 to Phenylalanine (V564F)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110108-plasmid-map-sequence-id-209785

    Enlarge

  19. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

    Enlarge

Showing: 1921 - 1940 of 2972 results