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pCIBN-EYFP-hLaminB1

Plasmid

#103800

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mRFP1-MP

Plasmid

#58367

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mRFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-TagRFP-SV40

Plasmid

#117805

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-EWSR1-gRNA

Plasmid

#237683

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWSR1 gRNA (EWSR1 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FcD265A-IL2

Plasmid

#65419

Purpose

Mammalian expression plasmid for murine serum-persistent IL-2

Depositor

Dane Wittrup

Article

Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

Insert

FcD265A-IL2 (Il2 Mouse)

Tags

His

Expression

Mammalian

Mutation

D265A

Promoter

Modified CMV Promoter

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
pcDHa_mDDX5_FL_K144N

Plasmid

#88870

Purpose

constitutive expression of Ha-DDX5 K144N in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Mutation

K144N

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT

Plasmid

#60419

Purpose

Mammalian Expression of ITSN DH/PH-tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

hITSN 1159-1509 // SSPB Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2

Plasmid

#237637

Purpose

For overexpression of mEGFP-SS18-SSX2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2 (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1a-mCherry-STIM1

Plasmid

#114176

Purpose

Gateway entry clone containing mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Gateway entry clone

Tags

mCherry (inserted after signal peptide)

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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