165,688 results

1. pLenti-PH-PLC-delta-EGFP

   Plasmid

   #136998

   Purpose

   Lentiviral EGFP lipid sensor for PI(4,5)P2

   Depositor

   Ken-Ichi Takemaru

   Insert

   PH-PLC-delta-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   EF1 alpha

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB2D

   Plasmid

   #195286

   Purpose

   Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR EGFP 2A Puro
   

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-4xTR-TUBE

   Plasmid

   #110312

   Purpose

   E. coli expression and purification of 4xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   4xTR-TUBE (UBQLN1 Human)
   

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S_GFP-GUS

   Plasmid

   #167122

   Purpose

   Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   GFP-GUS
   

   Tags

   mGFP5, GUS

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-PIK3CA

   Plasmid

   #116771

   Purpose

   Lentiviral expression of PIK3CA

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1801_pmax-dCasRx

   Plasmid

   #118634

   Purpose

   Transient transfection; Expresses dCasRx; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   dCasRx
   

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A

   Promoter

   CAGGS

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYTK001

   Plasmid

   #65108

   Purpose

   Part Plasmid Entry Vector to be used in the Dueber YTK system.

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   none
   

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBP-2_6.sensor

    Plasmid

    #162800

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6_RNA-device
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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