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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M

Plasmid

#140314

Purpose

Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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CHGA-eGFP

Plasmid

#228569

Purpose

Expresses GFP-tagged human Chromogranin A in mammalian cells

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

eGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA3

Plasmid

#153111

Purpose

Expression of Flag tagged human FOXA3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA3 (FOXA3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Brainbow4.2-PGK-HygTK

Plasmid

#214126

Purpose

Gene expression by Piggybac transposase in mammalian cells

Depositor

Sho Yoshimatsu

Insert

Brainbow

Expression

Mammalian

Mutation

Codon-optimized for mammalian cells

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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hHS1

Plasmid

#159169

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

hHS1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGFP-LacI

Plasmid

#179510

Purpose

Expresses EGFP-LacI in mammalian cells

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

LacI

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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