We narrowed to 2,710 results for: FAS

1. pJ414-PCY1:S495A

   Plasmid

   #98151

   Purpose

   Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:S495A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed serine 495 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ414-PCY1:W603A

   Plasmid

   #98153

   Purpose

   Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:W603A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tryptophan 603 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ414-PCY1:H695Q

   Plasmid

   #98154

   Purpose

   Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695Q
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to glutamine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ414-PCY1:H695A

   Plasmid

   #98155

   Purpose

   Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJ414-PCY1:Y696G

   Plasmid

   #98156

   Purpose

   Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:Y696G
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tyrosine 696 to glycine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZA3

   Plasmid

   #158481

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   L17E, STOP codon

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZA2

   Plasmid

   #158480

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   D489V, STOP codon

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZA11

   Plasmid

   #158489

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   L17E no STOP codon

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZA9

   Plasmid

   #158487

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   no STOP codon

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZA4

    Plasmid

    #158482

    Purpose

    Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D489V, STOP codon

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZA10

    Plasmid

    #158488

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    D489V, no STOP codon

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZA12

    Plasmid

    #158490

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D489V, no STOP codon

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNB682

    Plasmid

    #60143

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p(HA)HIF1alpha (P402A,P564A)

    Plasmid

    #52636

    Purpose

    mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

    Depositor

    Eric Huang

    Article

    Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

    Insert

    HIF1alpha P402A, P564A (HIF1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P402A, P564A

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Use

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

    Plasmid

    #158962

    Purpose

    Cre-dependent Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    hsp70l promoter
    floxed DsRed cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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