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PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

Plasmid

#102891

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas7

Plasmid

#106272

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasC

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas5

Plasmid

#106273

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasD

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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H1_sgRNA(GRIN2B)_SauCas9

Plasmid

#246054

Purpose

Expression of a sgRNA targeting the GRIN2B locus and SauCas9 under the same pol-III H1 promoter

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

SauCas9 and sgRNA(GRIN2B)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIC3_cpGR2(GGS5)-F59

Plasmid

#246053

Purpose

Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIC3_cpGR2-F59

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-2xmiR-122 target sites

Plasmid

#120302

Purpose

AAV vector for expression of AcrIIC1 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-2xmiR-122 target sites

Plasmid

#120303

Purpose

AAV vector for expression of AcrIIC3 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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dCas9-ddMSK1

Plasmid

#165603

Purpose

Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, Synthetic, S. Pyogenes)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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