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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

Plasmid

#118156

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CBFA2T3_GLIS2

Plasmid

#205786

Purpose

Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Expression

Yeast

Promoter

ADH1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-hSYN-meGFP

Plasmid

#225201

Purpose

Lentiviral construct expressing meGFP from human synapsin promoter.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

hSYN1

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9-UdgX

Plasmid

#163566

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pKG422 pBABE GFP-SPC24

Plasmid

#192696

Purpose

Retroviral transgene expression of human SPC24

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

SPC24 (SPC24 Human)

Use

Retroviral

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-N1

Plasmid

#54843

Purpose

Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Tags

mTurquoise2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pNL [NlucP/STAT5-RE/Hygro] Vector

Plasmid

#236819

Purpose

Express STAT5 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

STAT5 (STAT5A Human)

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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