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pMOD_C2906

Plasmid

#91093

Purpose

Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)

Use

Unspecified

Promoter

FMV 34S

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp6

Plasmid

#163590

Purpose

Expresses BMP-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp6 (Bmp6 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDSHA-SAR1-Myc

Plasmid

#67367

Purpose

Mammalian expression of hSar1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
KG#901

Plasmid

#110936

Purpose

Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SAR1-no stop

Plasmid

#67345

Purpose

hSar1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-Gdf5

Plasmid

#163596

Purpose

Expresses GDF-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf5 (Gdf5 Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-SAR1

Plasmid

#67472

Purpose

Mammalian expression of hSar1 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165887

Purpose

G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_1

Plasmid

#36395

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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B2375_CRY2olig-mEGFP

Plasmid

#215109

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-mEGFP (CRY2 Mustard Weed)

Tags

mEGFP

Expression

Mammalian

Mutation

E490G, 1-498

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT2A

Plasmid

#53109

Purpose

Expression of Drosophila 5-HT2A

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2A (5-HT2 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_4

Plasmid

#36334

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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As2

Plasmid

#123160

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR-ABS84, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-miR-34 MT

Plasmid

#78259

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated miR-34 target site

Use

Luciferase; Microrna activity

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CCGT to GG…

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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