We narrowed to 3,879 results for: luciferase expression
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Plasmid#140978PurposeEncodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaz-Rluc8 (GNAZ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai3-RLuc8
Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-eGFP
Plasmid#104834PurposeeFFly luciferase for invivo Imaging, eGFP for flourescent detectionDepositorInsertseFFly
eGFP
UseLentiviral and Luciferase; EgfpExpressionMammalianAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai2-RLuc8
Plasmid#140974PurposeEncodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai2-Rluc8 (GNAI2 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaGustducin-RLuc8
Plasmid#140979PurposeEncodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaGustducin-RLuc8 (GNAT3 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1
Plasmid#19132DepositorAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseExpressionMammalianMutationWTAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a-KRAB_crRNA NC
Plasmid#176262PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleasDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expreā¦TagsKruppel associated box domain and luciferaseMutationD917A and E1006A mutations to inactivate the endoā¦Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285
Plasmid#31060DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-285 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9-KRAB_sgRNA Td2
Plasmid#176264PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expreā¦TagsKruppel associated box domain and luciferaseMutationH840A and D10A mutations on spCas9 to inactivate ā¦Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_WT_ADRB2_LucReporter
Plasmid#129788Purposeencodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
ExpressionMammalianAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114099Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Luc-Sox2-3'UTR
Plasmid#63895PurposeLuciferase reporter of mouse wildtype Sox2 3'UTRDepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
ExpressionMammalianAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNL3.1-Erg
Plasmid#182706PurposeExpress Erg enhancer in mammalian cellsDepositorInsertEnhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
UseLuciferaseAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114104Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-257
Plasmid#31063DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-257 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMIR-CDH1 mutUTR
Plasmid#25039DepositorInsertCDH1 mutant 3'UTR (CDH1 Human)
UseLuciferaseExpressionMammalianMutationsubstitution of 6nt within the miR-9 binding siteā¦Available SinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2-hOct4 DE-SV40-Luc
Plasmid#26279PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorInsertOct4 Distal Enhancer Element (DE) (POU5F1 Human)
UseLuciferaseExpressionMammalianMutationSee Depositor Comments belowAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-164
Plasmid#31066DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-164 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_G102H_ADRB2_LucReporter
Plasmid#129794Purposeencodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorInsertADRB2 (ADRB2 Human)
UseSynthetic BiologyTags3x FLAGExpressionMammalianMutationG102HPromoterN/AAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom Ebox double mut
Plasmid#25854DepositorInsertDICER-Promoter Ebox double mut (DICER1 Human, gene ID 23405)
UseLuciferaseExpressionMammalianAvailable SinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom upstream Ebox Mut
Plasmid#25852DepositorInsertDICER-Promoter upstream Ebox Mut (DICER1 Human, gene ID 23405)
UseLuciferaseExpressionMammalianAvailable SinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-135
Plasmid#31067DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-135 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom downstream Ebox Mut
Plasmid#25853DepositorInsertDICER-Prom downstream Ebox Mut (DICER1 Human, gene ID 23405)
UseLuciferaseExpressionMammalianAvailable SinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)
Plasmid#114100Purposefusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Luc-Hdm4-3'UTR-F2d5
Plasmid#64018PurposeLuciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5DepositorInsertHDMX (MDM4 Human)
ExpressionMammalianAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-226
Plasmid#31064DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-226 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only