We narrowed to 3,747 results for: mak

1. pAAV.U6gT28.4.hSyn.H2B.RFP

   Plasmid

   #170387

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Promoter

   myo6b

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

   Plasmid

   #172506

   Purpose

   Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression vector with golden gate site for guides
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBLADE(FP6**)-mCherry

   Plasmid

   #168049

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Inserts

   Blue light-inducible AraC dimers in Escherichia coli
   mCherry
   

   Expression

   Bacterial

   Promoter

   J23101** and PBAD

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a-Con/Fon oG

   Plasmid

   #131778

   Purpose

   INTRSECT 2.0

   Depositor

   Karl Deisseroth

   Article

   Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

   Insert

   rabies glycoprotein - oG
   

   Use

   AAV; Intrsect

   Tags

   none

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IB-mGFP-FTH1

   Plasmid

   #192800

   Purpose

   Stable expression of mGFP-FTH1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   FTH1 (FTH1 Human)
   

   Tags

   monomeric EGFP (A206K)

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-ITGA6-1k-Basic-LUC

   Plasmid

   #107141

   Purpose

   Luciferase reporter plasmid to monitor transcriptional activity of canine ITGA6-promoter (1kb fragment)

   Depositor

   Aki Manninen

   Article

   Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

   Insert

   ITGA6-promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-TetON-Bmpr1A-ca

    Plasmid

    #217536

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    constitutively active BMP receptor 1a (BMPR1A Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet operon (TetO)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CaMKIIa-Flpo

    Plasmid

    #183535

    Purpose

    INTRSECT

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Has Service

    AAV8

    Insert

    Flpo
    

    Use

    AAV

    Mutation

    None

    Promoter

    CaMKIIa

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDF0317 HvsCas7-11 mammalian expression

    Plasmid

    #172511

    Purpose

    Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    HvsCas7-11 mammalian expression
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CaMKIIa-mScarlet

    Plasmid

    #131000

    Purpose

    AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Twitch-2B pRSETB

    Plasmid

    #48203

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIV-EF1a-2A-ZsGreen

    Plasmid

    #231985

    Purpose

    Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBLADE(FP6*)-mCherry

    Plasmid

    #168048

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Expression

    Bacterial

    Promoter

    J23101* and PBAD

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4-ATR-C

    Plasmid

    #53769

    Purpose

    mammalian expression of flag-tagged ATR C-terminal fragment

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal domain, aa 1398-2644*

    Promoter

    CMV

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGST2-GST-TEV-OPTN

    Plasmid

    #198428

    Purpose

    Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    optineurin (OPTN Human)
    

    Tags

    GST and TEV

    Expression

    Bacterial

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge