We narrowed to 17,391 results for: puro

1. pLentiCRISPR v2 puro mGSDME gRNA2

   Plasmid

   #223522

   Purpose

   Knocking out mGSDME in mouse cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCRISPR v2 puro mGSDME gRNA1

   Plasmid

   #223521

   Purpose

   Knocking out mGSDME in mouse cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

   Plasmid

   #195567

   Purpose

   PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-TRE-miRFP703_PGK-Puro

   Plasmid

   #216667

   Purpose

   Expresses tTa-inducible miRFP703, with puromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   miRFP703
   PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-N-Puro-mStayGold-TUBA1B

   Plasmid

   #227321

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTBL2368 MTK PB puro backbone

   Plasmid

   #226673

   Purpose

   Empty MTK PB cargo backbone marked with puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYX-N-Puro-mNeon-TSC22D1

   Plasmid

   #222667

   Purpose

   endogenously tag TSC22D1 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D1 (TSC22D1 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3puro-mGFP-FXR1a-Ccswap

   Plasmid

   #225460

   Purpose

   Express EGFP-fusion protein of FXR1 isoform a (CCswap)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CCswap (swapped positions of CC2 and CC1)

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3puro-EGFP-GAPDH-R rich

   Plasmid

   #225480

   Purpose

   Express mEGFP-GAPDH-Rrich fusion

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   GAPDH fused with R-rich region of TDRD3 (GAPDH Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3puro-mGFP-FXR1a-I304N

    Plasmid

    #225461

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a (I304N)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    I304N

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3puro-EGFP-GAPDH-Tudor

    Plasmid

    #225479

    Purpose

    Express mEGFP-GAPDH-Tudor fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3puro-EGFP-GAPDH-RGG

    Plasmid

    #225477

    Purpose

    Express mEGFP-GAPDH-RGG fusion

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH fused with RGG domain of TOP3B (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS puro 3XFLAG-hATXN7L3

    Plasmid

    #225725

    Purpose

    Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    ATXN7L3 (ATXN7L3 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS puro HA-hUSP27X S404N

    Plasmid

    #225724

    Purpose

    Transfection of USP27X (S404N) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X S404N (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S404N

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS puro HA-hUSP27X Y381H

    Plasmid

    #225723

    Purpose

    Transfection of USP27X (Y381H) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X Y381H (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y381H

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS puro HA-hUSP27X F313V

    Plasmid

    #225722

    Purpose

    Transfection of USP27X (F313V) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X F313V (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F313V

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS puro HA-hUSP27X K181E

    Plasmid

    #225721

    Purpose

    Transfection of USP27X (K181E) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X K181E (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K181E

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS puro HA-hUSP27X Y144C

    Plasmid

    #225720

    Purpose

    Transfection of USP27X (Y144C) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X Y144C (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y144C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS puro HA-hUSP27X G76S

    Plasmid

    #225719

    Purpose

    Transfection of USP27X (G76S) with HA tag in mammalian cells

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X G76S (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G76S

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHDR_NPM2-T2A-mGreenLantern-PuroTK

    Plasmid

    #222910

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge