We narrowed to 12,674 results for: sequence

1. pcDNA3-HA-CUL1 L756A/I757A

   Plasmid

   #19938

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 L756A/I757A (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   Mutation of two adjacent hydrophobic residues, L…

   Promoter

   Available since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pFX-Venus-KRasCT

   Plasmid

   #175834

   Purpose

   Express Venus with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIneo-EloC

   Plasmid

   #31982

   Depositor

   Joan Conaway

   Article

   Brower et al J Biol Chem. 1999 May 7;274(19):13629-36

   Insert

   Elongin C (ELOC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pUASp-FMW-attB-AthHen1

   Plasmid

   #104957

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   pTrasposase promoter

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sumo3 PolQM1 deltains3

   Plasmid

   #78459

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; delta 2497-2529

   Promoter

   T7

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pACYC-OspCas12c-noncoding

   Plasmid

   #120878

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-OspCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-TPI_WT-xrRNA-4MS2-4H

   Plasmid

   #108370

   Purpose

   Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PR594-His-bio

   Plasmid

   #52332

   Purpose

   Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PR594 (NSG1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.2 mir-1-1 reporter hsa-mir-138-2

   Plasmid

   #46677

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-138-2 (MIR138-2 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. RBMS3_2xRRM_pGEX

    Plasmid

    #135142

    Purpose

    Recombinant expression of RBMS3 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBMS3 (RBMS3 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. RBM24_RRM_pGEX

    Plasmid

    #135148

    Purpose

    Recombinant expression of RBM24 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM24 (RBM24 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCI-TPI_WT-4MS2-xrRNA-4H

    Plasmid

    #108371

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SGCD-His-bio

    Plasmid

    #52333

    Purpose

    Expresses full-length Delta-sarcoglycan ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SGCD (SGCD Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TXNDC-bio-His

    Plasmid

    #51858

    Purpose

    Expresses full-length Thioredoxin-related transmembrane protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TXNDC (TMX1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUASt-FMW-attB-AthHen1

    Plasmid

    #104958

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Hsp70 promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCELF PAI-RBP v3

    Plasmid

    #45507

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v3 (SERBP1 Rat, Human)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCELF PAI-RBP v2

    Plasmid

    #45505

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v2 (SERBP1 Rat, Human)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

20. P2RX6-bio-His

    Plasmid

    #53360

    Purpose

    Expresses full-length P2X purinoceptor 6 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    P2RX6 (P2RX6 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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