161,242 results

1. pLentiRNAGuide_003

   Plasmid

   #192505

   Purpose

   For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASTattB-2xFlag-mCherry-Msp300KASH

   Plasmid

   #170807

   Purpose

   Expresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesis

   Depositor

   Vikki Weake

   Article

   Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

   Insert

   2xFLAG/mCherry-Msp300KASH
   

   Use

   Tags

   2xFLAG-mCherry and Msp300KASH

   Expression

   Insect

   Mutation

   Promoter

   5xUAS + hsp70 minimal promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Has Service

   AAV6

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPTg.T2FpAXK (#52)

   Plasmid

   #35962

   Depositor

   Thomas Sato

   Article

   Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

   Inserts

   Tie2 promoter
   SV40 pA
   Tie2 enhancer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-mKelly1

   Plasmid

   #119782

   Purpose

   Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly1
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT899

   Plasmid

   #228288

   Purpose

   Constitutive mutant rPhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   rphlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

   Plasmid

   #44028

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P402A, P577A, N813A

   Promoter

   CMV

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJet-CMV-IARS1-GFP

   Plasmid

   #212322

   Purpose

   EGFP-tagged IARS1

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/Ndi-1/myc-His

   Plasmid

   #127503

   Purpose

   Expresses myc-tagged yeast Ndi-1 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   Ndi-1 (NDI1 Budding Yeast)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Hygro EYFP H148Q/I152L

    Plasmid

    #25874

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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