8,166 results

1. pGTy1mhis3-AI or pGTy1his3-AI

   Plasmid

   #62228

   Purpose

   A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

   Depositor

   David Garfinkel

   Article

   Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

   Insert

   Ty1H3 retrotransposon
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Feb. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pREP42-MCS+

   Plasmid

   #52691

   Purpose

   Same as pREP42, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Pnmt41

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTIN-ura4

   Plasmid

   #161808

   Purpose

   A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe

   Depositor

   Stewart Shuman

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Pho1

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

   Plasmid

   #116931

   Purpose

   Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   lumazine synthase
   

   Tags

   Sapphire

   Expression

   Yeast

   Promoter

   HIS3

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYAL_HLA-A2_MART1

   Plasmid

   #171177

   Purpose

   Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

   Depositor

   Michael Birnbaum

   Article

   Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

   Insert

   MART1_HLA-A2*01 single-chain trimer fusion
   

   Expression

   Bacterial and Yeast

   Promoter

   GAL1

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEF:ATP

   Plasmid

   #92179

   Purpose

   HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
   

   Use

   Integration and expression into yeast.

   Tags

   Yeast codon optimized mseCFP (donor) and cp173-mV…

   Expression

   Yeast

   Promoter

   TEF1p

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426-SNR52p-gRNA.CAN1.Y-SUP4t

   Plasmid

   #43803

   Purpose

   Encodes a gRNA that targets Can1.Y in yeast.

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   CAN1.y gRNA (CAN1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ960

    Plasmid

    #187972

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYZ462

    Plasmid

    #187970

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ959

    Plasmid

    #192603

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    ScTEF1

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Expression

    Mammalian and Yeast

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB655

    Plasmid

    #60142

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available Since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416-GAL-SUP35

    Plasmid

    #181705

    Purpose

    Galactose inducible expression of SUP35

    Depositor

    Alejandro Chavez

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG416-GAL-RNQ1

    Plasmid

    #181706

    Purpose

    Galactose inducible expression of RNQ1

    Depositor

    Alejandro Chavez

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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