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Addgene
Showing: 1 - 20 of 26354 results
  1. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pVHed07

    Plasmid
    #82350
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid
    Depositor
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pVHed02

    Plasmid
    #82352
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid
    Depositor
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
  5. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pGEX-6P-1-GST-CD(Cbx7)

    Plasmid
    #82525
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    CD(Cbx7) ; amino acids 1–62
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pGEX-6P-1-GST-ATL(Cbx7)

    Plasmid
    #82526
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    ATL(Cbx7), amino acids 66–81
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pGEX-6P-1-GST-CD-ATL (Cbx7)

    Plasmid
    #82527
    Purpose
    Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1–84
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid
    #82528
    Purpose
    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1-84 with mutation of RKR70-72, K75, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 26354 results