-
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Synthetic, Human)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPI/hPLK2WT/Neo
Plasmid#35385DepositorInserthPLK2 (PLK2 Human)
UseLentiviralTagsEMCV IRES-Neomycin cassette and V5 epitope and Po…ExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-2200
Plasmid#31062DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-2200 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsNRF2 (NFE2L2)
Plasmid#194303PurposeMammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTHDepositorInsertNFE2L2 (NFE2L2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pCAG-HA-NLGN3 R451C
Plasmid#59319PurposeExpression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutationDepositorInsertNLGN3 R451C (NLGN3 Human)
UseTagsHAExpressionMammalianMutationArginine 451 to Cysteine (R451C mutation)PromoterCAGAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight_A-CREB_PGK-tdTomato
Plasmid#154264PurposeDox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.DepositorInsertdominant negative CREB
UseTagsExpressionMammalianMutationPromoterTight/tetOAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry
Plasmid#179437PurposeDual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertcoreHS4-TRE-cit-coreHS4-mch (DC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB5
Plasmid#19473DepositorInsertDNAJB5 (DNAJB5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(ITSN1)
Plasmid#222631PurposeCdc42 activation using rapamycinDepositorInsertmCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-NUP37 neo
Plasmid#220361PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37DepositorInsertNUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA
Plasmid#218529PurposesgRNA targeting human EIF2AK1/HRIDepositorInsertEIF2AK1 (EIF2AK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
12x PRE TK luc
Plasmid#206163PurposeLuciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert12X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromotermin TKAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
XIAP (pBS0852)
Plasmid#185282PurposeFor the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.DepositorInsertXIAP (XIAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only