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PB-TetON-dual-SunTag-Hygro

Plasmid

#121119

Purpose

Endogenous gene activation with the SunTag CRISPR activation system

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

dCas9-GCN4x10_scFv-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

Plasmid

#66789

Purpose

Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D842V (constitutively active)

Promoter

EF1a

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only
CMV-MyoD

Plasmid

#8398

Depositor

Andrew Lassar

Article

Skapek et al Mol Cell Biol 1996 Dec;16(12):7043-53.

Insert

MyoD (Myod1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-mCD8

Plasmid

#162747

Purpose

NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-WT-mAbl

Plasmid

#69027

Purpose

Retroviral vector encoding WT mouse c-Abl

Depositor

Jean Wang

Article

Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

Insert

mouse Abl-Type IV (wild type)

Use

Retroviral

Tags

FLAG tag inserted at Internal SalI site (2934 bp)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 SIRT1-FL

Plasmid

#105670

Purpose

Mammalian expression construct of mouse SIRT1 full length isoform

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Giannino Del Sal, Licio Collavin

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTripz Ctrl-IRES-mCherry

Plasmid

#187295

Purpose

Doxycyclin inducible expression of ctrl IRES-mCherry

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

IRES-mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAd-CCN1

Plasmid

#12519

Depositor

Tong-Chuan He

Article

Si et al Mol Cell Biol. 2006 Apr . 26(8):2955-64.

Insert

CCN1 (Ccn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pKAM-E-Flag-GFP

Plasmid

#101866

Purpose

Lentiviral Evi-1-Flag with GFP marker

Depositor

Archibald Perkins

Insert

Evi-1 (Mecom Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2D

Plasmid

#32963

Purpose

For mammalian expression of mouse MEF2D with an HA tag

Depositor

Ron Prywes

Insert

MEF2D (Mef2d Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

E287G

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-mTRAF2

Plasmid

#174736

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Insert

TRAF2 (Traf2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Alpha1-Catenin-C-18

Plasmid

#53982

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Alpha1-Catenin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGSDME

Plasmid

#154877

Purpose

Expresses mGSDME in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Use

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCRY1 HA tag

Plasmid

#110298

Purpose

full length wt mouse Cry1 HA tag in pcDNA3.1

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

wt mcry1 HA tag (Cry1 Mouse)

Use

Tags

HA tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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