Showing: 41 - 57 of 57 results
-
Plasmid#123338PurposeMitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertDAKAP-Venus-cpVenus-FLARE-AKAR
UseTagsN-terminal 30 amino acids from DAKAP1, Venus, and…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Dronpa-FHA1
Plasmid#87711PurposeEncodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).DepositorInsertDronpa-FHA1
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PKAsub-TagRFPT-CAAX
Plasmid#87712PurposeEncodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub-TagRFPT-CAAX
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PKAsub(TA)-TagRFPT-CAAX
Plasmid#87740PurposeNegative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub(TA)-TagRFPT-CAAX
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-NES
Plasmid#138203PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-RLuc8N-NES
Plasmid#138212PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertPKAsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB1620 hU6-BpiI-Cas13bt3-DR
Plasmid#176315PurposehU6-BpiI-Cas13bt3-DR (crRNA expression)DepositorInsertCas13bt3 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB1679 CMV-HIVNES-GS-Cas13bt3
Plasmid#176317PurposeCMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)DepositorInserthuman codon optimized Cas13bt3
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB1676 REPAIR.t1
Plasmid#176319PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB1677 REPAIR.t3
Plasmid#176320PurposeCMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB1923 REPAIR.t1-S
Plasmid#176321PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q/E620G/Q696L, only the deaminase domain (aa …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6AvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 57 of 57 results