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Addgene
Showing: 141 - 160 of 4509 results
  1. pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-198}:NosT

    Plasmid
    #212172
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  2. pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-216}:NosT

    Plasmid
    #212173
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (216 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  3. pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-390}:NosT

    Plasmid
    #212174
    Purpose
    This binary vector expresses mNeonGreen containing the PIV intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PIV-390}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PIV int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  4. pLSU1/t35s[TMV]:mNeonGreen-CI{AGO-198}:NosT

    Plasmid
    #212175
    Purpose
    This binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{AGO-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and AGO int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  5. pLSU1/t35s[TMV]:mNeonGreen-CI{PDK-198}:NosT

    Plasmid
    #212176
    Purpose
    This binary vector expresses mNeonGreen containing the catalase (PDK) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
    Depositor
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
  6. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid
    #126478
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Inserts
    Or47a (Or47a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  7. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and T7
    Availability
    Academic Institutions and Nonprofits only
  10. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
  11. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
  12. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
  13. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 4509 results