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Showing: 1 - 20 of 57 results

  2. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194470-plasmid-map-sequence-id-384877

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  3. pC0037

    Plasmid
    #181934
    Purpose
    Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    Gaussia luciferase
    Cypridina luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1alpha
    Availability
    Academic Institutions and Nonprofits only
    181934-plasmid-map-sequence-id-369337

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  20. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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Showing: 1 - 20 of 57 results