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Showing: 21 - 40 of 264 results

  8. P75ECD-VfAU1-LOV_448

    Plasmid
    #63633
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by the extracellular and transmembrane domain of p75NTR and a restriction site
    Depositor
    Harald Janovjak
    Insert
    P75ECD-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63633-plasmid-map-sequence-id-105954

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  9. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. II801: pMVP (L5-L4) ecDHFR DD

    Plasmid
    #121721
    Purpose
    pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    ecDHFR Degron Domain (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121721-plasmid-map-sequence-id-237132

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  15. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  16. mVenus-P75ECD-VfAU1-LOV_458

    Plasmid
    #63634
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by mVenus, the extracellular and transmembrane domain of p75NTR and a restriction site
    Depositor
    Harald Janovjak
    Insert
    mVenus-P75ECD-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63634-plasmid-map-sequence-id-105955

    Enlarge

Showing: 21 - 40 of 264 results