Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1 - 20 of 48 results

  5. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Use
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

    Enlarge

  14. RG-001

    Plasmid
    #133439
    Purpose
    Heat 3 domain of human elF4G in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4G1 (EIF4G1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133439-plasmid-map-sequence-id-269573

    Enlarge

  15. pSW149

    Plasmid
    #122248
    Purpose
    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.
    Depositor
    Sarah Walker
    Article
    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18
    Insert
    TIF4631 (TIF4631 Budding Yeast)
    Use
    Tags
    Intein-Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    122248-plasmid-map-sequence-id-245052

    Enlarge

  17. pLNHA-C1-HseIF4G_O

    Plasmid
    #147087
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147087-plasmid-map-sequence-id-289534

    Enlarge

  18. pT7-EGFP-C1-HseIF4G_O

    Plasmid
    #147091
    Purpose
    Mammalian Expression of HseIF4G
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4G (EIF4G1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation M432V compared to the seu…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147091-plasmid-map-sequence-id-421630

    Enlarge

Showing: 1 - 20 of 48 results