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pAc5.1B-lambdaN-HA-DmeIF4Ga-4Ebind-mut-dsRNAres_Q

Plasmid

#147272

Purpose

Insect Expression of DmeIF4Ga-4Ebind-mut-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmeIF4Ga-4Ebind-mut-dsRNAres (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutaion K340R compared to the sequ…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga-del205-234-dsRNAres_Q

Plasmid

#147273

Purpose

Insect Expression of DmeIF4Ga-del205-234-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmeIF4Ga-del205-234-dsRNAres (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutaion K340R compared to the sequ…

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G +MIC

Plasmid

#158790

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Use

Tags

3xFlag and BirA*

Expression

Mammalian

Mutation

Promoter

miniCMV

Availability

Academic Institutions and Nonprofits only

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C150

Plasmid

#174350

Purpose

Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eIF4G1

Use

In vitro transcription template

Tags

FLAG

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H

Plasmid

#146493

Purpose

Insect Expression of DmeIF4Ga-Nterm

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmeIF4Ga-Nterm (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmeIF4G_578-650-GB1_Z

Plasmid

#148154

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmeIF4G_578-650_Z

Plasmid

#148183

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence comp…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I

Plasmid

#146577

Purpose

Insect Expression of DmeIF4Ga

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K340R compared to the seq…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-DmCup_417-1133_T

Plasmid

#147589

Purpose

Insect Expression of DmeIF4Ga_578-650-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga_578-650-DmCup_417-1133 (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-eIF4G delN83

Plasmid

#65812

Purpose

expression of eIF4G-MS2 fusion.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

eIF4G (EIF4G1 Human)

Use

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

delN83 (functionally behaves as the WT protein)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_571-676-GB1_AG

Plasmid

#148745

Purpose

Bacterial Expression of HseIF4G_571-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_571-676 (EIF4G1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_592-653_AG

Plasmid

#148746

Purpose

Bacterial Expression of HseIF4G_592-653

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_592-653 (EIF4G1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

Plasmid

#79259

Purpose

Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmeIF4G (eIF4G1 Fly)

Use

Schneider 2 cell expression

Tags

V5-His6 and lambdaN-HA

Expression

Mutation

Promoter

Ac5 promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-675-GB1_AD

Plasmid

#148504

Purpose

Bacterial Expression of DmeIF4G_578-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-675 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4G_601-634-GB1_AD

Plasmid

#148505

Purpose

Bacterial Expression of DmeIF4G_601-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-634 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_601-660-GB1_AD

Plasmid

#148506

Purpose

Bacterial Expression of DmeIF4G_601-660

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-660 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge