Showing: 21 - 40 of 140 results
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Plasmid#184094Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT22cUm5En2SE4P3Che(#1189)
Plasmid#184095Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2E4P3Che(#1208)
Plasmid#184096Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutation5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …Promoter5xUASAvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En2SE4P3Che(#1209)
Plasmid#184097Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasm5En25EE4P3Che(#1213)
Plasmid#184099Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …Promoter5xUASAvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 2A
Plasmid#183640PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 35 and 40 of PEN2 mutated to alaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pReg-FEN2
Plasmid#190616PurposeExpresses a generalist biosensor responsive to bicyclic monoterpenes in E. coliDepositorInsertFEN2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-4
Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
psen2_L (OZ577)
Plasmid#35217DepositorInsertZinc finger array targeting psen2 (psen2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
psen2_R (OZ578)
Plasmid#35218DepositorInsertZinc finger array targeting psen2 (psen2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 20A
Plasmid#183639PurposeLentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 D90A
Plasmid#183641PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 90 of PEN2 mutated to alaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hLAMP2-C-PEN2
Plasmid#183646PurposeLentiviral expression of a lysosome-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hTOMM20-C-PEN2
Plasmid#183647PurposeLentiviral expression of a mitochondrion-residential PEN2.DepositorUseLentiviralTagsExpressionMutationWTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 140 results