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pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGluSnFR3.v857.PDGFR

Plasmid

#178336

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGluSnFR3.v857.SGZ

Plasmid

#178337

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGluSnFR3.v857.GPI

Plasmid

#178338

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-GFP

Plasmid

#178580

Purpose

Expression of GFP under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-mCherry

Plasmid

#178581

Purpose

Expression of mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CFAP410_pcDNA6.2/EmGFP-Bsd

Plasmid

#176948

Purpose

Mammalian expression vector encoding CFAP410 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CFAP410 (C21orf2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R]

Plasmid

#177512

Purpose

Mammalian Expression Plasmid of anti-GFAP (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFAP (Homo sapiens) recombinant mouse monoclonal antibody (GFAP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_FAP site with scFv

Plasmid

#188351

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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GFAP-Archon-EGFP

Plasmid

#187979

Purpose

Expresses the fluorescent voltage indicator Archon under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Archon1-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).cpSFGFP.HaloTag

Plasmid

#209662

Purpose

Non-responsive control

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al bioRxiv 2023.08.24.554624

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

GFAP

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CFAP52:1-620

Plasmid

#211235

Purpose

Mammalian expression of full-length human CFAP52. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CFAP52:M1-S620 (WDR16 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

A91V C199S E336K annotated sequence conflicts

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CFAP52:1-620

Plasmid

#211309

Purpose

Mammalian expression of full-length human CFAP52. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CFAP52:M1-S620 (WDR16 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

A91V C199S E336K annotated sequence conflicts

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SV40.eGFP.SV40(polyA)

Plasmid

#195558

Purpose

Expresses eGFP under control of short GFAP promoter

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

eGFP

Use

AAV

Tags

No tags

Expression

Mammalian

Mutation

Promoter

short GFAP

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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GFAP scFv [N206A/8]

Plasmid

#199426

Purpose

Mammalian expression of GFAP scFv. Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFAP (Human) (GFAP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIG-747_CD19-28z_CAR_TFAP4

Plasmid

#207489

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-28z_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-1]

Plasmid

#206703

Purpose

Mammalian Expression Plasmid of anti-GFAP (Human). Derived from hybridoma N206A/8-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFAP (Homo sapiens) recombinant Mouse monoclonal antibody (GFAP Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-2xNLS-mTurquoise2

Plasmid

#104053

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

GFAP

Availability

Academic Institutions and Nonprofits only

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