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Showing: 21 - 40 of 68 results

  1. pT7-MS2-HA-C1-HsYTHDC2_1069-1430_AD

    Plasmid
    #148492
    Purpose
    Mammalian Expression of HsYTHDC2_1069-1430
    Depositor
    Elisa Izaurralde
    Insert
    HsYTHDC2_1069-1430 (YTHDC2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L1409Q annotated as natur…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    148492-plasmid-map-sequence-id-290940

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  4. pAAV-HDC-DIO-GFP-shRNA.scramble

    Plasmid
    #184331
    Purpose
    Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
    Use
    AAV, Cre/Lox, Mouse Targeting, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    histidine decarboxylase promoter fragment that g…
    Availability
    Academic Institutions and Nonprofits only
    184331-plasmid-map-sequence-id-365459

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  5. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

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  7. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

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  8. pAAV-iCre-2A-cherry

    Plasmid
    #182246
    Purpose
    Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Availability
    Academic Institutions and Nonprofits only
    182246-plasmid-map-sequence-id-355958

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  11. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  14. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

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  15. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  17. pD649-HAsp-CLEC4A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156564
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CLEC4A (CLEC4A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Availability
    Academic Institutions and Nonprofits only
    156564-plasmid-map-sequence-id-368735

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  19. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

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  20. p416-GPD-DIPP2-HA

    Plasmid
    #183947
    Purpose
    Expresses human HA-tagged DIPP2 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP2 (NUDT4 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Availability
    Academic Institutions and Nonprofits only
    183947-plasmid-map-sequence-id-363373

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Showing: 21 - 40 of 68 results