Addgene

Showing: 1 - 14 of 14 results

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pSGE_763_4xUAS_UPS_hkCP

Plasmid

#71171

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

763_4xUAS_UPS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

DSCP Drosophila synthetic core promoter

Availability

Academic Institutions and Nonprofits only

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Epac-S-H159

Plasmid

#170347

Purpose

mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

Insert

mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Epac-S-H189

Plasmid

#170350

Purpose

mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

Insert

mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Kv1.1/RBG4

Plasmid

#196943

Purpose

Plasmid expressing an antigen targeting the Kv1.1 potassium channel subunit

Depositor

James Trimmer

Insert

Kv1.1 (Kcna1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPERPluc1

Plasmid

#14810

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec . 1(14):1048-57

Insert

PERP promoter (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec . 1(14):1048-57

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-huHkCas12a

Plasmid

#174683

Purpose

Expresses 6xHis-MBP tagged HkCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huHkCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Mc4r->M71-IRES-tauGFP ACNF TV

Plasmid

#105072

Purpose

Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassette

Depositor

Peter Mombaerts

Article

Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.

Insert

Mc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.1 K+ channel [K20/78R]

Plasmid

#128616

Purpose

Mammalian Expression Plasmid of anti-Kv1.1 K+ channel (Rat). Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.1 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcna1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#182065

Purpose

Mammalian Expression of Kv1.1 K+ channel scFv. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv1.1 K+ channel (Rattus norvegicus) (Kcna1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#190486

Purpose

Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.1 K+ channel [K20/78R-2b]

Plasmid

#206623

Purpose

Mammalian Expression Plasmid of anti-Kv1.1 K+ channel (Rat). Derived from hybridoma K20/78-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv1.1 K+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Kcna1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.1 K+ channel [K20/78R-1]

Plasmid

#206681

Purpose

Mammalian Expression Plasmid of anti-Kv1.1 K+ channel (Rat). Derived from hybridoma K20/78-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv1.1 K+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Kcna1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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