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Addgene
Showing: 261 - 280 of 3943 results

  7. pLT237

    Plasmid
    #174300
    Purpose
    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.
    Depositor
    Lee Lynd
    Article
    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1
    Insert
    PolC homology arm
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Cysteine 669 to Tyrosine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174300-plasmid-map-sequence-id-392933

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  18. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  20. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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Showing: 261 - 280 of 3943 results