Showing: 21 - 40 of 62 results
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Plasmid#39305DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationK148A, K150A and K151APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)
Plasmid#39307DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationT249A, D250A and E251APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)
Plasmid#39302DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationD88A, K89A, D94A and K95APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)
Plasmid#39303DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationQ109A, W110A, K112A and K113APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)
Plasmid#39304DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationK122A, D123A, Y124A and D125APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))
Plasmid#39308DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationC-terminal 7 aa (QEEQEEY) removedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)
Plasmid#39300DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationS16A, R17A, H18A, D19A, W23A and N25APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)
Plasmid#39306DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationE211A, K212A, E213A, R214A, D215A and Y217APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEB1deltaC-mCherry
Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorInsertEB1 (MAPRE1 Human)
UseTagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-mCherry-6xHis
Plasmid#130984PurposeBacterial expression of mouse EB1-mCherry-6xHis for protein purification.DepositorInsertEB1-mCherry-6xHis (Mapre1 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Zdk1-EB1C
Plasmid#107695PurposeC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsZdk1 and mCherryExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Zdk1-EB1C
Plasmid#107696PurposeC-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsEGFP and Zdk1ExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDEST/N1-hEB1-GFP
Plasmid#27382DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 62 results