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Addgene
Showing: 141 - 160 of 196 results

  4. pcDNA3.3_CoV2_501V2

    Plasmid
    #170449
    Purpose
    Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    170449-plasmid-map-sequence-id-333042

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  5. pcDNA3.3_CoV2_P1

    Plasmid
    #170450
    Purpose
    Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    170450-plasmid-map-sequence-id-333035

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  6. pCDNA3.3_CoV2_B.1.1.7

    Plasmid
    #170451
    Purpose
    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    170451-plasmid-map-sequence-id-333038

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  10. pKG123

    Plasmid
    #63074
    Purpose
    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-C (CENPC Human)
    Use
    Retroviral
    Tags
    TEV-S and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63074-plasmid-map-sequence-id-135907

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  11. pLNHA-C1-HsDCP1a_131-582_L

    Plasmid
    #146813
    Purpose
    Mammalian Expression of HsDCP1a_131-582
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_131-582 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T1716G compared to the sequen…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    146813-plasmid-map-sequence-id-289260

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  12. pT7-EGFP-C1-HsDCP1a_1-132_O

    Plasmid
    #147089
    Purpose
    Mammalian Expression of HsDCP1a_1-132
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_1-132 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T237C compared to the sequenc…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147089-plasmid-map-sequence-id-289536

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  13. pT7-EGFP-C1-HsDCP1a_132-582_O

    Plasmid
    #147090
    Purpose
    Mammalian Expression of HsDCP1a_132-582
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_132-582 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T1716G compared to the sequen…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147090-plasmid-map-sequence-id-289537

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  15. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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Showing: 141 - 160 of 196 results