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Addgene
Showing: 1 - 20 of 79 results
  1. pWPI-myogenin

    Plasmid
    #79073
    Purpose
    Lentiviral plasmid that constitutively expresses human myogenin
    Depositor
    Insert
    human myogenin (MYOG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Availability
    Academic Institutions and Nonprofits only
  2. pINDUCER20-hMYOG-3xHA (#733)

    Plasmid
    #78342
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYOG ORF with a 3xHA tag
    Insert
    MYOG (MYOG Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Availability
    Academic Institutions and Nonprofits only
  3. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. hMYOG-3xHA-IRES-hrGFP (#324)

    Plasmid
    #78341
    Purpose
    Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Insert
    MYOG (MYOG Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. TFORF3362

    Plasmid
    #144838
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    MYOG (MYOG Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  8. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    Insert
    Mb 64Y68F
    Use
    Tags
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 79 results