We narrowed to 17 results for: nek7

1. pDONR223-NEK7

   Plasmid

   #23395

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. NEK7_HUMAN_D0

   Plasmid

   #79735

   Purpose

   This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-N-HA-NEK7

   Plasmid

   #75142

   Purpose

   Expresses N-terminal HA-tagged NEK7 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. LT3GEPIR-NEK7shRNA1

   Plasmid

   #220550

   Purpose

   To inducibly knockdown NEK7 expression

   Depositor

   Sean Lee

   Insert

   NEK7 shRNA1 (NEK7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LT3GEPIR-NEK7shRNA2

   Plasmid

   #220551

   Purpose

   To inducibly knockdown NEK7 expression

   Depositor

   Sean Lee

   Insert

   NEK7 shRNA2 (NEK7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. NEK7 gRNA (BRDN0001162196)

   Plasmid

   #77740

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK7 (NEK7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NEK7 gRNA (BRDN0001145539)

   Plasmid

   #77739

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK7 (NEK7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NEK7 gRNA (BRDN0001149173)

   Plasmid

   #77741

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK7 (NEK7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p667-UBC-NEK7-V5-miniTurbo_IDG-K

    Plasmid

    #189882

    Purpose

    Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-N-Flag-NLRP3

    Plasmid

    #75127

    Purpose

    Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge