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Addgene
Showing: 121 - 140 of 170 results
  1. pR1G108CP20X

    Plasmid
    #85086
    Purpose
    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
    Depositor
    Insert
    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pPD2cX

    Plasmid
    #86968
    Purpose
    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Insert
    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pPD1cR

    Plasmid
    #86970
    Purpose
    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Insert
    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, G108C in PCNA1
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  5. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  6. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  8. p3R112C/T180CHFm

    Plasmid
    #85091
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
    Depositor
    Insert
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of …
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pPD2X

    Plasmid
    #86967
    Purpose
    Expresses PCNA2 protein fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Insert
    PCNA2 fused to phosphite dehydrogenase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 170 results