Addgene

Showing: 21 - 40 of 83 results

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pRL-TK let7 G

Plasmid

#11330

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation G from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 H

Plasmid

#11331

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation H from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation I from figure 4A of paper.

Promoter

Availability

Academic Institutions and Nonprofits only
prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK 4x mut A

Plasmid

#11314

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation A (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut B

Plasmid

#11315

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation B (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut C

Plasmid

#11316

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation C (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut D

Plasmid

#11317

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation D (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut E

Plasmid

#11318

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation E (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation F (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut G

Plasmid

#11320

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation G (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation H (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut I

Plasmid

#11322

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation I (see paper) on inner 2 binding sites.

Promoter

Availability

Academic Institutions and Nonprofits only
pRL_CT_GI_del1

Plasmid

#98744

Purpose

For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Inserts

phytochrome interacting factor 3 (PIF3 Mustard Weed)
phytochrome B (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

SV40-NLS, VP64-AD, and synTALE-DBD

Expression

Yeast

Mutation

mutated nucleotide 576 C to A, deleted amino acid…

Promoter

FBA1 and TDH3

Availability

Academic Institutions and Nonprofits only

Enlarge
pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

Plasmid

#184563

Purpose

CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

All lysines were mutated to arginines. Codon-opti…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL3_1-169_C104D

Plasmid

#209797

Purpose

Bacterial expression of human PRL-3 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
CyPet-A206K-PRL2

Plasmid

#149685

Purpose

For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

Depositor

Motoyuki Hattori

Article

Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

Insert

CyPet-A206K-PRL2

Use

Tags

8 histidine tag

Expression

Bacterial

Mutation

CyPet A206K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only