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Showing: 1 - 20 of 136 results

  2. Tol2-GgVCL/WT

    Plasmid
    #162787
    Purpose
    CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    beta actin
    Availability
    Academic Institutions and Nonprofits only
    162787-plasmid-map-sequence-id-327628

    Enlarge

  7. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172837-plasmid-map-sequence-id-340906

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  8. Tol2-GgVCL/*TBM

    Plasmid
    #162790
    Purpose
    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    Use
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    changed Gly 454 to Val
    Promoter
    beta actin
    Availability
    Academic Institutions and Nonprofits only
    162790-plasmid-map-sequence-id-321532

    Enlarge

  12. pEGFPC3/GgVcl 884-1066 (Vt)

    Plasmid
    #46272
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (Vt) (VCL Chicken)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    884-1066 (Vt) tail domain
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    46272-plasmid-map-sequence-id-65165

    Enlarge

  13. pET15b/GVcl 1-1066 /*TBM

    Plasmid
    #162781
    Purpose
    amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Gly 454 to Val
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162781-plasmid-map-sequence-id-321530

    Enlarge

Showing: 1 - 20 of 136 results