We narrowed to 142 results for: vcl
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Plasmid#110045PurposeProtein expression and purification of VCL_VinculinDepositorInsertVCL_Vinculin (VCL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/WT
Plasmid#162787PurposeCONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsIRES-GFPExpressionBacterial and MammalianMutationWTPromoterbeta actinAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vclb
Plasmid#185411PurposeGFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin b (vclb Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 (Vh)
Plasmid#46268DepositorInsertVcl 1-851 (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-851 (Vh) head domainPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-1066
Plasmid#46265DepositorInsertVcl (VCL Chicken)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
UseTagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC3/GgVcl 884-1066 (Vt)
Plasmid#46272DepositorInsertVcl 884-1066 (Vt) (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation884-1066 (Vt) tail domainPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 1-851
Plasmid#46264DepositorInsertVcl 1-851 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 1-851 (aka Vh) head domainPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl V1-851 (aka Vh)
Plasmid#46172DepositorInsertVcl 1-851 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 (aka Vh) head domainPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 1-258
Plasmid#46263DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleInsertGallus gallus vinculin (VCL Chicken)
UseTagsExpressionBacterialMutationchanged Gly 454 to ValPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-1066 T12 mutant
Plasmid#46266DepositorInsertVcl T12 mutant (VCL Chicken)
UseTagsEGFPExpressionMammalianMutationT12 mutant (D974;K975;R976; R978A) autoinhibition…PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
GgVcl 1-1066 FRET tail probe 1
Plasmid#46276DepositorInsertVcl 1-1066 + EYFP (VCL Chicken)
UseTagsECFPExpressionMammalianMutationaddition of 9 bp linker and EYFP at aa 883PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorInsertVcl (VCL Chicken)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 (aka VD1)
Plasmid#46270DepositorInsertVcl 1-258 (aka VD1) (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-258 (VD1 domain)PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 A50I mutation
Plasmid#46271DepositorInsertVcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-258 (VD1 domain) A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 A50I mutant
Plasmid#46269DepositorInsertVcl 1-851 A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-851 (Vh) head domain, A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only