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Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)

Pooled Library

#1000000049

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Feng Zhang

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_1_2

Pooled Library

#187249

Purpose

Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
RLC11

Pooled Library

#163955

Purpose

Targets open reading frames and non-coding RNAs in M. smegmatis.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83969

Purpose

Version 2 of the human CRISPRi genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
TCR Rapid Assembly for Functional Testing (TCRAFT)

Pooled Library

#1000000264

Depositor

Michael Birnbaum

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83978

Purpose

Version 2 of the human CRISPRa genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
Human sgRNA library Brunello in lentiCRISPRv2

Pooled Library

#73179

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
Human sgRNA library Brunello in lentiGuide-Puro

Pooled Library

#73178

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
MORF Library Kit with GFP and mCherry controls

Pooled Library

#1000000218

Depositor

Feng Zhang

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only
The Human Druggable CRISPR library

Pooled Library

#200013

Purpose

Knockout library containing ~10,000 sgRNAs targeting 1,980 human druggable genes (5 sgRNAs per gene and 100 non-targeting control sgRNAs).

Depositor

Lianxin Liu

Article

Zheng et al Adv Sci (Weinh). 2023 Aug;10(22):e220679

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiGuide-Puro

Pooled Library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)

Pooled Library

#1000000048

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Feng Zhang

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only
Phage ORFeome

Pooled Library

#249629

Purpose

This library consists of approximately 400 phage open reading frames (ORFs), each cloned into an IPTG-inducible vector.

Depositor

Aaron Whiteley

Article

Nagy et al Nat Microbiol. 2026 Feb;11(2):597-609. d

Expression

Bacterial

Species

M13, Ms2, T2, T7, and Λ

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only
Human Transporter Knockout library

Pooled Library

#213695

Purpose

Next generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Ferdigg et al Life Sci Alliance. 2024 Nov 22;8(2):e202

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only
Mouse SLC KO Library

Pooled Library

#141436

Purpose

Knockout library targeting mouse SLC proteins.

Depositor

Giulio Superti-Furga

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)

Pooled Library

#1000000111

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

John Doench, David Root

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only
Human CRISPR Metabolic Gene Knockout Library

Pooled Library

#110066

Purpose

Targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Use

Lentiviral

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Gouda in pRDA_118 backbone

Pooled Library

#136987

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
Human SAM library in lentiSAMv2 backbone

Pooled Library

#1000000078

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 400 results

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More Filters

400 results

Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)

hCRISPRa_dual_1_2

RLC11

hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

TCR Rapid Assembly for Functional Testing (TCRAFT)

hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Human sgRNA library Brunello in lentiCRISPRv2

Human sgRNA library Brunello in lentiGuide-Puro

MORF Library Kit with GFP and mCherry controls

The Human Druggable CRISPR library

Mouse sgRNA library Brie in lentiGuide-Puro

Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)

Phage ORFeome

Human Transporter Knockout library

Mouse SLC KO Library

Toronto KnockOut version 3 library (TKOv3)

Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)

Human CRISPR Metabolic Gene Knockout Library

Mouse sgRNA library Gouda in pRDA_118 backbone

Human SAM library in lentiSAMv2 backbone

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