388 results

1. Membrane protein gRNA library

   Pooled Library

   #113345

   Purpose

   Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform.

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

2. PB-EF1a-Puro-V8.2

   Pooled Library

   #178943

   Purpose

   Barcoding library for the macsGESTALT inducible lineage recording system

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Expression

   Mammalian

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

3. hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

   Pooled Library

   #1000000091

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   Jonathan Weissman

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pSMAL-CellTag-multi-v1 barcode library

   Pooled Library

   #206045

   Purpose

   The CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal tracking

   Depositor

   Samantha Morris

   Article

   Jindal et al Nat Biotechnol. 2023 Sep 25. doi: 10.103

   Use

   Lentiviral

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

5. hCRISPRi_dual_1_2

   Pooled Library

   #187246

   Purpose

   Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

6. Barcode Library 0 (BCL0)

   Pooled Library

   #179851

   Purpose

   Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

   Depositor

   Jason Moffat

   Article

   Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

   Expression

   Mammalian

   Use

   Lentiviral

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

7. CROPseq-iT7 Human CRISPR Knockout Pooled Library (Brunello)

   Pooled Library

   #223064

   Purpose

   Knockout library to carry out genome-scale optical screening using Nuclear In-Situ Sequencing (NIS-Seq).

   Depositor

   Jonathan Schmid-Burgk

   Article

   Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

8. LARRYv2-mScarlet library 1

   Pooled Library

   #233697

   Purpose

   Barcoding pooled library

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

9. Human CRISPRi sgRNA library Dolcetto in backbone XPR_050

   Pooled Library

   #1000000114

   Purpose

   Genome-wide CRISPR gRNA pooled library for inhibition of human genes

   Depositor

   John Doench, David Root

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

10. Human Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187080

    Purpose

    Library targeting 2,989 human metabolic genes for activation of gene expression.

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

11. Human CRISPR Metabolic Gene Knockout Library

    Pooled Library

    #110066

    Purpose

    Targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Use

    Lentiviral

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

12. p11-5’_10xN_PAM-site3 (RTW572)

    Pooled Library

    #160134

    Purpose

    Plasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Species

    Synthetic

    Use

    CRISPR

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Barcode Library 1 (BCL1)

    Pooled Library

    #179852

    Purpose

    Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

    Depositor

    Jason Moffat

    Article

    Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

    Expression

    Mammalian

    Use

    Lentiviral

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

14. SPLINTR mCHERRY v1

    Pooled Library

    #179775

    Purpose

    SPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Expression

    Mammalian

    Use

    Lentiviral

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

15. Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer

    Pooled Library

    #101926

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

16. Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility

    Pooled Library

    #101931

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A

    Pooled Library

    #92385

    Purpose

    Crispri

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Use

    Lentiviral

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

18. hCRISPRa_dual_1_2

    Pooled Library

    #187249

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

19. Mouse sgRNA library Gouda in pRDA_118 backbone

    Pooled Library

    #136987

    Purpose

    Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library.

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

20. Bassik lab Mouse CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility

    Pooled Library

    #1000000126

    Purpose

    CRISPR gRNA mouse knockout pooled library - Trafficking, mitochondrial, and motility

    Depositor

    Michael Bassik

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only