We narrowed to 990 results for: plasmid dna

1. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73178-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pGP-AAV-syn-jGCaMP8m-WPRE (AAV1)

   Viral Prep

   #162375-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Ef1a-Coff/Fon-oScarlet (AAV8)

   Viral Prep

   #137138-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-oScarlet (#137138). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-oScarlet plasmid DNA. EF1a-driven, Flp-dependent expression of oScarlet (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   oScarlet (Flp-dependent)

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

   Viral Prep

   #137130-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   BFP (Cre-dependent)

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-syn-Flpo (AAV Retrograde)

   Viral Prep

   #174378-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-syn-Flpo (#174378). In addition to the viral particles, you will also receive purified pAAV-syn-Flpo plasmid DNA. Syn-driven expression of the recombinase FLPo. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Promoter

   Tags

   None

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

6. hSyn-DIO-somBiPOLES-mCerulean (AAV5)

   Viral Prep

   #154951-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   Syn

   Tags

   mCerulean (Cre-dependent)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-EF1a-Flpo (AAV Retrograde)

   Viral Prep

   #55637-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Tags

   None

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

   Viral Prep

   #137144-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   mCherry (Flp-dependent)

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hSyn-GRAB_NE1h (AAV9)

   Viral Prep

   #123309-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1h (#123309). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1h plasmid DNA. hSyn-driven expression of GRAB-NE1h norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Promoter

   Syn

   Tags

   None

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Ef1a-mCherry-IRES-Cre (AAV8)

    Viral Prep

    #55632-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a and Ef1a/IRES

    Tags

    mCherry

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-EF1a-Cre (AAV Retrograde)

    Viral Prep

    #55636-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a

    Tags

    None

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV PHP.eB)

    Viral Prep

    #44361-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (AAV8)

    Viral Prep

    #137161-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (#137161). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    oScarlet (Cre-dependent)

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

14. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral Prep

    #73179-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Promoter

    Tags

    None

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (AAV1)

    Viral Prep

    #101060-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (#101060). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Promoter

    Syn

    Tags

    None

    Available since

    July 23, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)

    Viral Prep

    #131004-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    None

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pGP-AAV-syn-jGCaMP8f-WPRE (AAV1)

    Viral Prep

    #162376-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Syn-ChrimsonR-tdT (AAV5)

    Viral Prep

    #59171-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-Ef1a-Con/Fon-mCherry (AAV8)

    Viral Prep

    #137132-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    mCherry (Cre and Flp-dependent)

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

20. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV Retrograde)

    Viral Prep

    #87306-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Promoter

    EF1a

    Tags

    None

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only