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We narrowed to 677 results for: RON

Showing: 1 - 10 of 677 results

  1. The SGC Toronto Collection

    Type
    Collection
    ... from the Structural Genomics Consortium (SGC) Toronto collection...Genomics Consortium Plasmids Browse SGC Toronto collection Structural Genomics Consortium Plasmids Structural...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags ...

  2. AAV for Neuronal Tracing

    Type
    Collection
    ...Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools for Monosynaptic Neuronal Tracing These... tracing from single, genetically targeted neurons. Neuron. 53(5):639-47. PMID: 17329205 Don’t See What...service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies virus...with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies...rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...that it could only infect a genetically specified neuronal population. This is done using the envelope protein... pseudotyping the rabies virus with EnvA, only neuronal cells engineered to express TVA will be infected...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...Jan-Willem Veening Christopher Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering...plasmids related to intercellular signaling and environmental sensing. Strains Browse Addgene's collection...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia nigra, ..., which is the main source of the body’s DA. Neurons often fire at a certain frequency, and this frequency...understood, the direct effect of DA binding to a target neuron is more mysterious. Thus, scientists have developed...dynamics of optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling ...brain slices Demonstrate DA detection in a single neuron in vivo in Drosophila Detect DA dynamics in response...PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed genetically...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...the brain to visualize how neurons are connected. Viral Vectors as Neuronal Tracers Due to their ability...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...Converting a transsynaptic neuronal tracer into a monosynaptic (first order) neuronal tracer For RABV to crawl...nerve terminals of adjacent neurons (i.e., it is taken up by projection neurons). Research into these phenomenon...order neurons, via entry mediated by the G protein/G-protein receptor. Once in first order neurons, the...most of the projections to these new neurons came from nearby neurons. It wasn’t until later time points...mapping of direct inputs to midbrain dopamine neurons. Neuron. 2012 Jun 7;74(5):858-73. Pubmed PMID 22681690...

  7. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...available types of degron tags. What is a degron tag and why should I use one?   Degron tags are a class...Finally, some degron tags can do more than just degrade! Read on to find out more. Common degron tags When... comes to degrons, you have options! Below we will review the 4 most common types of degrons and some ...protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...the protease and degron remain attached to the tagged protein. In this event, the degron domain shuttles...In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously...expressed proteins. Here we will review what advantages degron tags can offer over other protein control methods...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...Limitations of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent...experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures GFP...label somata but not neuronal processes. In contrast, smFPs can label fine neuronal structures with high...functions and others are controlled by collections of neurons that are intricately wired into circuits through...have recently made it much easier to map these neuronal networks. For almost three decades, recombinant... in order to define those that target specific neuronal populations in the brain. The Allen Institute ...quickly find the best AAV serotypes to target the neurons they’re studying. Thanks to this work it is now...

  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...parts of the brain is mediated via individual neurons. Neurons are composed of a cell body, with dendrites...proximal neuronal cells. In the specific example of episodic memory, a subset of hippocampal neurons projects...axons directly to the prefrontal cortex, but also indirectly via synapses to neurons in other brain regions...using these viruses to mark neuronal connectivity a large majority of neuronal cells are labeled overtime...auditory neuronal circuitry in vivo. A variant of RABV has been developed to only label a neuronal connection...AAV vectors can be equipped with strong promoters for high and neuron-specific transgene expression. Fluorescent...cell types, for example only dopaminergic neurons, neuronal tracing has the added benefit of only labelling...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...bidirectional promoters, or the creation of bicistronic or multicistronic vectors. Unlike promoters which will...upstream cistron will have a few additional residues added to the end, while the downstream cistron will ...transcripts for each gene that is expressed, multicistronic vectors simultaneously express two or more ... so in this blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work... and how to get started with them. Why Use multicistronic vectors? Detecting cells that are expressing... selectable markers from a unique promoter, multicistronic plasmids ensure that any cells that are positive...derived from the same transcript. Of course multicistronic vectors do not have to exclusively be used ...
Showing: 1 - 10 of 677 results