Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 73 results for: 4-Oct

Showing: 1 - 50 of 73 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Encoded Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. Gary Yellen NADH/NAD+ Peredox fluorescent...interneurons across vertebrate species. Nat Neurosci. 2016 Oct 31. Gordon Fishell Calcium GCaMP5 genetically encoded...indicator for neural activity imaging. J Neurosci. 2012 Oct 3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren...marking active neuron populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression...intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn2+ FRET... Secretion of Pancreatic Islets. Anal Chem. 2019 Oct 1;91(19):12212-12219. Huiwang Ai Zinc GZnP2 Cytosolic... for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf Frommer Pyruvate Pyronic...

  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...help decrease the time to your first manuscripts. 4. Balance focus and exploration Researchers face a ...have finite time and resources. You typically have 4-5 years to make discoveries and publish papers to ... of your postdoctoral training experience. If you're going to commit to doing a postdoctoral fellowship...contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia Research Campus of the...the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face a number of challenges ranging...academia - the main reason most people enter postdoctoral training. Similarly, there are several factors...1. Reverse engineer success On Day 1 of your postdoctoral experience, define what will be needed to achieve...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...between Rab12 and LRRK2 . Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries...target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural oxytocin receptors using an AAV-CRISPR...dopaminergic activity in vivo. Sun et al. Nat Methods. 2020 Oct 21. Glutamate indicators with improved activation... Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs ....transmission. Aggarwal et al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ... pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain...iPS cells; retroviral expression of human Sox2, Oct3/4, Klf4, and c-Myc from four separate plasmids Induction...transient expression of human L-Myc, Lin28, Sox2, Klf4, Oct3/4, Glis1, and EBNA1 from separate plasmids An Efficient...polycistronic expression of human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert...Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53, Klf4, Sox2, L-Myc, Lin28 from separate ...iPS cells; retroviral expression of mouse Sox2, Oct3/4, Klf4, and c-Myc from separate plasmids Induction...Apr 14;4(4):727-43. Woltjen Plasmid Mouse Non-integrating polycistronic expression of mouse Oct4, Klf4, ...

  5. p53 Pathway

    Type
    Collection
    ...p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097 . The expanding...Menendez D, Inga A, Resnick MA. Nat Rev Cancer. 2009 Oct;9(10):724-37. PubMed PMID: 19776742 . Germline TP53...cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C....Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390/v13102073 Resources ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...

  7. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Other citations include...Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495. PMID: 27759036 Rosario, et al. 2016. ... entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID: 31611639 AAV2(trpYF) When using the...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028. PMID: 4878700...Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. ... formation of plasmid multimers. Cell. 1976 Dec;9(4 PT 2):707-16. doi: 10.1016/0092-8674(76)90134-3. PMID...in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406. PMID: 4884687...capillary gel electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense...plasmid monomerization and stability. Cell. 1984 Apr;36(4):1097-103. doi: 10.1016/0092-8674(84)90060-6. PMID...upstream production. Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/j.biotechadv.2009.02.003. Epub...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ....2013.08.021. Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using...2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library...Jan;33(1):102-6. doi: 10.1038/nbt.3055. Epub 2014 Oct 19. PubMed . In vivo genome editing using Staphylococcus... Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014....Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed . Return to GeCKO...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...determine optimal antibiotic concentration. Last Update: Oct. 27, 2017 Protocol Video Watch the protocol video...Prepare carbenicillin to a concentration of 1 mg/mL – 4 mg/mL in LB medium. The concentration of antibiotic... 1 mg/mL plate and effective selection. 150 µL of 4 mg/mL Carbenicillin plated over-agar Plate shows several...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi: 10.1016/j.ijpharm.2018.08.043...:3499-3510. doi: 10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley...absorption coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct;72(10):8150-7. PubMed PMID: 9733856. PubMed Central...PMID: 25531807. PubMed Central PMCID: PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR...Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...heavy diversity 4-23 (non-functional) IGHD423 IGHD4-4 immunoglobulin heavy diversity 4-4 DA4, IGHD44 IGHD5...heavy variable 4-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304...immunoglobulin heavy variable 4-39 IGHV439, VH IGHV4-4 immunoglobulin heavy variable 4-4 IGHV44, VH IGHV4-59 immunoglobulin...104A BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944, MGC118945, hBD-4 DEFB4 defensin, beta 4 DEFB-2...lambda variable 4-3 IGLV43, V5-1 IGLV4-60 immunoglobulin lambda variable 4-60 IGLV460, V5-4 IGLV4-69 immunoglobulin...hIL-3Ra IL4 interleukin 4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402 IL4R interleukin 4 receptor CD124, IL4RA ...

  17. Genetic Code Expansion

    Type
    Collection
    ...Thomas Huber 207620 pRSF-G1(4/5FTrp)RS G1(4/5FTrp)RS Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp...jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram 182884 AzoRS-4 tyrosyl-tRNA...4CNW)RS G1(4CNW)RS Methanogenic archaeon 4-Cyano-L-Tryptophan (4-CN-Trp) Bacterial TAG Thomas Huber 222872... Methanogenic archaeon S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine...translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery...acid would be incorporated. Other options, such a 4-base pair codons, have also been utilized. A tRNA ...pDule-IBBN (G2) IBBN (G2) synthetase M. jannaschii 4-(2′-bromoisobutyramido)-phenylalanine (IBBN) and structurally...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...FEBS Lett 2012 Oct 19;586(20):3716-22. Park et al., Science 2011 Aug 26;333(6046):1151-4. pOSIP and...transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation... Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84...assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the number of...Stigleoclonium helveticum (ShChR), Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas...

  21. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016/j.xgen.2024.100519..., vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021... Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015...

  22. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...447–455 . https://doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X...

  23. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning...blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing when compared...

  24. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1 ...is the expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation ...mixture of two vectors that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen...and remains a safety concern in the clinical field [4]. Three major reprogramming methods are considered...PMID: 21243013. PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal...activated T cells and Sendai virus. Nat Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S.,...Mukherjee. Kusumika (Kushi) Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with...

  25. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ... specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency: When a ...zygote through to the morula stage are totipotent [2-4]. Pluripotency: When a cell has the ability to differentiate...pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed PMID: 18295576. PubMed Central...PMID: 19343304. PubMed Central PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective...fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174. 9. Takahashi, ... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

  26. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...function in the environment where it will localize [4]. Most investigators are surprised to learn that FPs...biology."Current Protocols in Cell Biology (2005): 21-4. Pubmed PMID: 18228466. Pubmed Central PMCID: PMC2875081...Pubmed PMID: 6526011. Pubmed Central PMCID: PMC557822. 4. Costantini, Lindsey M., and Erik Lee Snapp. "Fluorescent...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... from Albert Einstein College of Medicine. Her doctoral research was conducted in the laboratory of Erik...subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina at...

  27. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...only and leave exon 2 floxed, as seen in line 4 of Figure 4. Since the loxP sites are located in intronic...the targeted exon in the gene of interest (Figure 4). When recombination occurs, the gene still functions...has been placed into an intron.    Figure 4: A targeting vector to create a floxed allele for ... protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure...

  28. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ... single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate ...Framework for in vivo T cell screens. J Exp Med, 221(4), e20230699. https://doi.org/10.1084/jem.20230699.... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...

  29. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174. 4. Yu, J., et al., Induced pluripotent...delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation...pancreatic insulin-producing cells. Cell Res, 2009. 19(4): p. 429-38. PubMed PMID: 19255591. 17. Spence, J....converting one cell into another. Cell Stem Cell, 2008. 3(4): p. 382-8. PubMed PMID: 18940730. Additional Resources...involves overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

  30. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...futures both inside and outside of academic research (4). There are currently 17 sites around the USA undertaking...PMID: 22567149. Pubmed Central PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to ...the Future of Research will be held from 22-24 October. This blog has been contributed by guest blogger...strong supporter of increasing the number of postdoctoral scientists at University of Maryland. Paula... can register here or live stream the event on October 22nd and 23rd here. Many thanks to our guest blogger...

  31. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...board, Jacqueline N Crawley  [et al]. 2013;4(435):4 35 1-4  20. doi: 10.1002/0471142301.ns0435s65. PMID: ...for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn about the ...animal models. Nature reviews Neuroscience. 2012;13(4):251-66. doi: 10.1038/nrn3171. PMID: 22430017. 2. ....2011.10.003.  PMID: 22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  32. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...intermediate on the path to the formation of cysteinyl-tRNA (4). The discovery of a phosphoserine (Sep)-tRNA synthetase...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes amber...letters 462.3 (1999): 302-306. PubMed PMID: 10622715. 4. Sauerwald, Anselm, et al. "RNA-dependent cysteine...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...blogger Natalie Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research...

  33. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...Cell Stem Cell 4, no. 6 (2009): 472-76. PubMed PMID:19481515. PubMed Central PMC: 2705327  4. Hockemeyer,...reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID: 21339765. Methods figures...PubMed PMID: 20139967. PubMed Central PMCID: 2892897. 4. Stadtfeld, M., and K. Hochedlinger. "Induced pluripotency...a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles...expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus...plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if...

  34. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were first... Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni,...contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types...with their environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department...

  35. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic...Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17. 10.1016/j.chembiol.2012.02.006. 2 Guntas...expression of cDNAs and shRNAs with a reporter gene, or (4) multiple sgRNAs with Cas9 for genome engineering....Sladitschek HL and Neveu PA. PLoS One. 2015. Apr 24;10(4):e0124958. LOC8-ing mitochondrial fusion with a...system was also designed for a short screening time (<4 hours) in order to avoid complicating effects from...improving the strength of the promoters used; and 4) optimizing expression of both the light sensor and...The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to characterise the ...

  36. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...and potentiates the effect of PD-1 blockade.“ (Ref 4). PD-1 helps tumor cells evade the immune system -...356.6338 (2017): eaal3118. PubMed PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent...Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics...

  37. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function upon...immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at Binghamton University. She works ...

  38. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...people have regular schedules. Work 8 to 5 or 7 to 4 or maybe 7 to 6 and 8 to 10 or whatever works for ...carefully and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

  39. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...spread (metastasize) and turned into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate... is large in size and may have spread while Stage 4 indicates that the cancer has spread from where it... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

  41. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...optogenetic implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23042500; PubMed Central PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  42. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    ...of 39 Scientific Impact Measures" (2009) PLOS One 4(6): e6022. doi: 10.1371/journal.pone.0006022 Aragon...been gaining ground. A study issued by Google in October entitled “Rise of the Rest: The Growing Impact ...

  43. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...38 kb Cells HEK-293T Sf9 Time to collection 2–4 days 5–10 days Purification Ultracentrifugation ...

  45. Tetracycline Inducible Expression

    Type
    Collection
    ...effectors for Tet transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link...TetO-FUW-OSKM Tet-On inducible expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation Rudolf...-tetO-hOKMS Tet-On inducible expression of human Oct4, Sox2, Klf4, and Myc for iPS cell generation Tarjei...

  46. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...PMID: 19396156. PubMed Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP...and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed PMID: 26853302. PubMed Central PMCID...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

  47. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...might be contextual fear conditioning (reviewed in [4]). Contextual fear conditioning is a behavioral experiment...PMID: 23473324. PubMed Central PMCID: PMC3595120. 4.Mattis, Joanna, et al. "Principles for applying optogenetic...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  48. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...buffer. Additionally, protein lysis should be done at 4 °C or on ice, whichever is more practical. If your...protein-based blockers degrade fairly quickly, even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies...temperature and time. Though the most common conditions are 4 °C overnight or room temperature for 1–2 hours, there...protein/sample loaded Incubate primary antibody at 4 °C overnight Incomplete washing Ensuring wash...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...

  49. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...grant writing Job changes can happen regularly (every 4-6 years is common), but this can also bring new opportunities—projects...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

  50. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161-022-00046-4 Kumar, S. R., Miles, T... fold change in RNA and DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail...nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086...106–115. https://doi.org/10.1038/s41593-021-00969-4 Goertsen, D., Goeden, N., Flytzanis, N. C., & Gradinaru...enhanced transgene expression in the macaque CNS. Med, 4(1), 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002.... R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer, J., Winchester...
Showing: 1 - 50 of 73 results