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We narrowed to 191 results for: CO

Showing: 1 - 50 of 191 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...to only four days (Paix et al., 2017). The co-CRISPR and co-conversion approaches have the advantage of...using a co-injection marker that is mainly lost in F2 animals, removing the need to segregate away co-conversion...knock-ins at other genomic loci. The first approach – co-CRISPR from Craig Mello’s lab – used inactivation...oligo-mediated knock-in of a dominant mutation, known as co-conversion (Arribere et al., 2014). In both cases...amounts of experimental manipulation and screening.  A co-selection strategy for CRISPR editing in C. elegans...approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair of a conditional-lethal...center images depict the power of lethal mutation co-selection. Only animals rescued for the conditional...

  2. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...expect from a co-op experience.  Sarah Gordon is a rising junior at Northeastern and Addgene co-op studying... to complete a six month work experience called a co-op to receive a degree. Northeastern compiles a list...position at Addgene as a marketing and social media co-op. There are a lot of different worries that come... made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It may have been... “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked about my current...building out social media spreadsheets every day of my co-op. I had to do research on loads of different best...

  3. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...chosen field. This cooperative education experience (co-op) is what has brought me, a second year bioengineering...first saw the comprehensive job description for this co-op, I was immediately interested in applying. This...accepted that day and was excited for the start of my co-op in January. This excitement did not come without...shows how they fit into the system. Specifically as a co-op, it is encouraged to explore different teams and.... She is one of several students completing their co-op at Addgene. This is her second contribution to...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...scientists have instead developed novel methods to co-express your gene along with a reporter, such as ...in the same cell. Although it is possible to drive co-expression by using a plasmid with multiple, individual...another ribosome recruitment site, thereby resulting in co-expression of two proteins from a single mRNA. IRES...efficiency. How do I get started? If you are looking to co-express your gene of interest along with a fluorescent...Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression of multiple unique genes, you can start...

  5. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...to become a product. I spoke with Dr. Mike Koeris, co-founder and current VP of Business Development & ...from academia to a startup Mike Koeris and fellow co-founders Tim Lu and others first came up with the...skills necessary to be a succesful entrepreneur? As a co-founder of a biotech startup, Mike explained, his...self-sustainable. Within a year from this decision, Mike and his co-founders raised $6 million. The biggest challenge...Beckman Acknowledgments Thank you to Dr. Mike Koeris (Co-founder and formerly VP Operations of Sample6) for...

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...more than one vector to express the gRNAs and Cas9. Co-transfections can lead to variable expression levels...expressed from a polycistronic transcript  Requires co-transfection of Cas9 Nissim et alTsai et al PTG...expressed from a polycistronic transcript Requires co-transfection of Cas9   Xie et al  Multiplexing...most of Zetsche et al’s experiments, a single vector co-expressing Cpf1 and a crRNA array were used.  With...the targeted brain region and ~75% of neurons were co-transduced with Cpf1 and GFP.  Of these neurons, ...

  7. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Fan, Kenneth Fan, and Benjie Chen! Melina Fan is a co-founder of Addgene. Melina received her Ph.D. in ...earned a B.S in Biology from MIT.   Kenneth Fan is a co-founder of Addgene. Kenneth received his Bachelor...Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder of Addgene and received his PhD in Computer...

  8. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive...contributions of each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene ...

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...double-strand break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  10. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ... a graduate student at Harvard University, former Co-director of Science in the News, and member of the...received her PhD from Harvard University, is a former Co-director of Science in the News, and is a member ...

  11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...is Lauren Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern...interview series was born. Over the six months of my co-op, I have conducted informational interviews with...Staying true to your values While going through the co-op application process, my advisor encouraged me ... project was one of the biggest takeaways from my co-op. It has been so insightful to learn about different...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...fused the Cas enzyme to cell-penetrating peptides and co-incubate it with an “assist peptide” that facilitates...viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide (AP). C) Workflow ...Workflow for Cas-RNP-PAGE editing of primary T cells, by co-incubation of Cas-RNP and assist peptide. Adapted... brains with brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...genome loci, the most efficient and easiest way is to co-transfect several plasmid together, with each plasmid...clone two spacers for each locus into PX330, then co-transfect or delivery the two plasmids together into...use double nickase, you need to have four spacers co-expressed and using PX335 to express the Cas9n (nickase... test run them, and then select the best guide to co-transfect in HR donor. Alternatively, a new system...10 µM oligo stock for each well on 24-well plate, co-deliver into the cells with the Cas9/sgRNAs vector...screened? A13: Before starting your experiment, consider co-transfecting with GFP. This allows you to sort for...puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR/Cas system with your homologous...

  14. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...clinical development programs in biopharma. AveXis, co-founded by Kaspar, acquired by Novartis and rebranded...the mutation (Le et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors...syndrome program being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous technical.../10.1002/1096-8628(200022)97:2<147::aid-ajmg6>3.0.co;2-o Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... family members, and professional network such as co-workers, professors, and mentors. Ask for specific...economically disadvantaged populations. Roo is the co-founder of STEAMid.       Rose C. St Pierre is currently...help their children thrive academically. Rose is the co-founder of STEAMid.     Additional resources on the...

  16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...editor is fused to a fast-splicing split intein and co-infected into the cell. Intein splitting removes ...1: The base editor is split into two plasmids and co-transfected into cells for reconstitution. Image ... permission. Though the lab suspected that the co-transduction by two viruses would lower editing efficiency...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  18. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... a graduate student at Harvard University, former Co-director of Science in the News, and member of the...received her PhD from Harvard University, is a former Co-director of Science in the News, and is a member ...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... her brother Ken Fan, and her husband Benjie Chen co-founded Addgene in 2004. They worked tirelessly to...the Room or Habitat for Humanity take you and your co-workers out of the office and into a completely new...mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut! We’ve done everything...

  22. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  23. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment...from the same incompatibility group should not be co-transformed. +Actual copy number varies. See below...

  25. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK...

  26. CRISPR Plasmids - Yeast

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  27. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein...

  28. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs at BWH and providing...is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle Program at BWH, and...health strategy, and global health equity. She is also Co-founder/director of the non-profit foundation IMFAHE...

  29. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

  30. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

  32. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation...termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes...

  33. Worm Expression Resources

    Type
    Collection
    ...Link opens in a new window) Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing...Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...

  34. Antibodies 101: Isotypes

    Type
    Blog Post
    ... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ...

  35. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and...

  38. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify ...ClonTracer to study drug resistance Although Bhang and co-workers started using the tool to look at a variety...

  39. Working with Nuclear Receptors

    Type
    Blog Post
    ...steady-state complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex...group of proteins, whereas the more loosely bound co-coregulators associate with the core complex in a...

  40. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for...

  41. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to...

  43. CRISPR References and Information

    Type
    Collection
    ...neuronal co-injection marker); pCFJ104 (body wall muscle co-injection marker); pCFJ90 (pharyngeal co-injection...

  44. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough...packaged in them and use elements such as IRES or 2A to co-express them from one promoter. Specificity The ...

  46. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  47. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...system, Cas9 is tagged with a biotin acceptor site and co-expressed with BirA biotin ligase. The locus is isolated...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...

  49. dTAG - You're it!

    Type
    Blog Post
    ...that bind an E3 ligase. These bifunctional degraders co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau...refer to as the degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery...

  50. Addgene at SfN23!

    Type
    Blog Post
    ...workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and ...
Showing: 1 - 50 of 191 results