We narrowed to 933 results for: KIN

...less phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs...fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest...proxy for kinase activity.  Advantages of KTRs When compared to FRET reporters for measuring kinase activity...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing...phenotype. One method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging...Covert Lab provides an alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization...

...am currently working on projects for the Science Communication function at Addgene. Working a 9-5 As it...one semester (the majority spend two to three) working in your chosen field. This cooperative education...bioengineering student, to Addgene. I am currently working on the QC/QA team as a DNA Sequencing Intern. ...interview was intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved...environment on my team and at Addgene, I felt comfortable asking questions without fearing they would be disregarded...addition to these core hours, we have flexible working hours. For Addgene that means that it is possible...

...story. This year, we made significant strides in making science more accessible and reliable. We continue...flexibility. But this is just the beginning. We’re working toward giving you more choice in how you receive... every breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank...

... such as nutritional status. Within the animal kingdom, this information is relayed through factors (both...such as genes involved in metabolism, growth or cytokine action). Primary or core coregulators (those that...cellular roles. In the current phase of NURSA we are seeking to compliment these consortium-generated datasets...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

...side conversations within the team. We are not talking about gossip here – this means encouraging a culture...Connie Cepko to learn about her management style.   Seeking feedback frequently One of the most important paths... only way.   From a colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead...

...Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...subset of kinases. The Problem:  There is no common method for creating optically controlled kinases. The ...design for creating photoswitchable kinases and use MEK1 as a model kinase. In this system, light is used ...photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar ...photoswitchable kinases good for? There are a number of potential applications for photoswitchable kinases.  Below...Zhou et al. 1. Testing kinase inhibitors Zhou et al examined the ability of kinase inhibitors to alter Raf1... by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal resolution...

...as-is, saving time and effort but risking the potential of them not working, or spend a few weeks designing...If you’ve ever been looking for just the right CRISPR vectors on Addgene and found instead ones that ... the heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right...right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction digest...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...Fragmid website.   If the vector is to your liking, your last step, Step 4, is to give the assembled...six or seven components — in most cases, that's making two or more plasmids — it may be more efficient...

...stories, and other 20th anniversary celebrations, by checking out our blog, social media, newsletter, and website...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-...mission and work. In 2024, we will be actively seeking ways to support a global community where every ...the Addgene blog 2022 and 2023: Reflections and Looking Forward...

...jobs where all I did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify...breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking as a scientist...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an...support for your ideas, invite others into decision making processes, and even interview better! As a scientist...science communicator, I’ve done a lot of public speaking, and I'm happy to share what I've learned along...When my mind went blank, my options were to stop talking until I could present something coherent or to ...me accomplish my goal.   As a scientist, public speaking isn't a performance art. You’re (usually) presenting...

...accomplishments from 2022 and share what we’re looking forward to in 2023 and beyond. Last year, we distributed...rapidly changing environment. In particular, we are working to understand what challenges remain in materials...changing landscape of science. We are therefore embarking on a strategic refresh to understand this new ...help a great number of people. I look forward to working with Addgene colleagues, collaborators, partners...

...relationships because networking has gotten a bad reputation (as in…”I just hate networking”). Scientists enjoy... having to talk with strangers. Be bold in asking all kinds of people to connect with you and to share...the nonacademic sphere where most of us will be working.  Check out Joanne's Reddit AMA It should be no...fine to ask about specific jobs (that is what networking is about), but be upfront about interest in a... yes and they might say no but do not confuse networking for a job connection with ongoing relationship...relationship building through Informational Interviews. Making Your informational interviews productive Informational...remote conversations. In this interview you will be asking the questions, so be prepared. There are many good...

...when you can, instead of just asking for favors.  Don’t focus on networking with “important” or senior people...helpful who you’d like to get to know better. Networking is not about how many contacts you have; it’s...become a volunteer). Naturally running into or working with the same people is often the easiest way to...get a response. It breaks an important law of networking--don’t be creepy. There is a person on LinkedIn...they don’t know me. It’s a waste of our time.  Networking connections can last years This is a graphic ... the Addgene blog Browse other blog posts on networking Attending a conference soon? Get tips here Learn...

...as coming from networking at the time, but that means it was the best kind of networking! Since nearly ... parents looking for experts to come give guest presentations. If you’re a scientist thinking about reaching...was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was...my community. This past year, I spent some time thinking about how I could combine all of these roles by...space, time events, and network for volunteers. Speaking of volunteers, you can’t run an event like this...call to local parents who would feel comfortable speaking on topics that would compliment the STEM curriculum...been aimed at grad students, postdocs, and other working scientists, creating a presentation for 8th graders...

...gene expression of cells at a single time point, making it difficult to investigate how early expression...and cell identity at the single-cell level, thus linking ancestral gene expression patterns with reprogramming...reprogramming outcomes (Biddy et al., 2018). Tracking lineage and cell identity at the single-cell level with...re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path. The CellTags revealed that...intestine, demonstrating its utility in long-term tracking in vivo (Guo et al., 2019). Key takeaways of CellTag...cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics of gene expression and clonal...cells or the clonal evolution of cancer. If you’re looking to use CellTag, you can find the CellTag libraries...

...definitely recommend checking out GCE4All’s website and resources, and then checking out all of the plasmids...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...reagents!) for the use of GCE. In short, they’re working to make GCE available for all biomedical researchers...

...Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you... events will generate these types of mutations, making it an ‘easy’ editing job to get done. However, ...observed when inserts are within 10 bps of the break, making the cut off essentially as part of the templated...

...Streaking for single colonies is an integral part of any bacteriologist’s skill set. So when Dave Westenberg... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave... suspension,” says Westenberg. In addition to streaking for single colonies, these chromoprotein-expressing...

...founded in 1944 with the goal of facilitating networking between chemistry students, especially those ...scientific disciplines, as well as social and networking events. Imperial Synthetic Biology Society, ... synthetic biology by providing education and networking opportunities for society members as well as ...in society and to support iGEM alumni-industry networking to promote the creation of start ups and biotech...students and the general public, career events, and networking meetups. The society hopes to help young scientists...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn... networking...

...of cells. Looking ahead What’s next, you might wonder? Verkhusha says they are always looking to improve... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... and intensity allows for optical labeling and tracking of proteins, organelles, and living cells in a...improve upon existing genetically-encoded probes by making them brighter, more photostable, smaller, and less... the 750 to 800 nm range, and his team is also looking in the direction of biosensors capable of revealing... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...

...about replying and thinking about good questions and answers. Professional networking groups will often...Check out Joanne's Reddit AMA Who should I be looking for? Be on the lookout for friends or colleagues...who gives a great talk, ask them for advice on speaking. If you see someone with a great professional ...t doing it at all yet) watch my webinar, “Not Networking 101-Building Relationships for Success”. Choose...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good...to organize a successful peer mentoring circle. Asking someone to be your mentor Mentoring is a “loaded...

...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...contributed by guest blogger, Dalila Cunha de Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick...Running Smoothly and "Gnome-Proof" This is why the “Bricking Science” YouTube channel was born. The name came...with each other through globalized, collaborative thinking. LEGO and science: The potential is endless One...feet), to connect people with science. And so, “Bricking Science” was born. We chose the stop motion format...approachable for LEGOs. Stop motion consists of taking many pictures, one for each position in a movement...video takes an entire week of our free time. After taking almost a thousand pictures, my husband Enzo will...

...2 Here! We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

...group in industry? The collaborative nature of working in industry and how delegating can empower people...she sets metrics for success. The importance of thinking about the big picture and not just the next experiment...Chapter 6: Advice to scientists who are planning on working in industry. The importance of recognizing and ...

...genome randomly, while others use attP sequence docking sites so that you can control exactly in which ...at different loci. Fortunately, there are many docking sites available for integration. 2. How soon would...would know beforehand on which chromosome your docking site is located). This amounts to a small difference...important to note so that you know what to look for. Making your transgenic fly and downstream experiments ...experimentation. Drosophila are amenable to many different kinds of experimental assays depending on your interests...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...Central PMCID: PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis...

...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...that. Now you have the basic knowledge to start working with flies. Like Addgene, the Drosophila community...chromosomes to use. If you’re not ready to start working with live animals yet, Harvard’s DRSC/TRiP- Functional...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...has been supported by a Steering Committee and working groups with representatives from all areas of the...

...here and you can find more tips and protocols for working with lentivirus on our blog and protocol pages.... love to hear from you if you have used or are thinking about using our viral services. Please send any...

...mouse and human kinomes. While any subset of the genome is limited by definition, the kinome is a well-annotated...genetic screening project can be a tremendous undertaking, producing a wall of results that can only be... About CRISPR Pooled Library Screening Why the kinome? While properly designed perturbations – in the...incremental approach is prudent. What's in the CRISPR kinome library? Thus, we are glad to provide CRISPR libraries...pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed with either 4 or 8...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs found... Additionally, this set can be used for cherry-picking individual gRNAs that score as hits in pooled screens...

...need to be cost and time efficient. We do this by making our own competent cells and using a little-known...efficiency of transformation (for example, if you are working with a library or an especially low concentration...will work just fine.  A number of protocols for making competent cells are available online. A few examples...

... I found Vectr to be fairly intuitive and I was making figures quickly. Inkscape Inkscape is the most...images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search...

...fluorescent proteins to oligomerize is known as “stickiness.” Since the fluorescent tag should operate as... per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration...oligomerization in cells. In addition to measuring stickiness, several researchers have also collected data...characteristics are important, but have less priority than stickiness. In my experience, fluorescent proteins that ...independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed ... measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in single cells." PloS one 11.1 (2016): e0146789...

...really fit in anywhere and everyone who was doing the kind of work my lab wanted to do already seemed to know...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...This was invaluable to me as a bench scientist working at an institution where training opportunities ...network at the meeting. We found our mentors by asking for help with the program on social media. Many...between mentors and Singletons can accelerate the networking timetable for the Singletons in their bins.  ...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...leaps. Binning Singletons is its own organization, working to make scientific meetings more productive for...

...differently if I would be speaking to a colleague in the office next door. So be kind, helpful, and thorough...

...University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate ... YFG. You could compare this mutant fly to one lacking the mutation in the same genetic background. In...express YFG in a new tissue type (more details on making a fly line in the next blog post). For example,...get the right cell type expression as opposed to making a new fly with hedgehog under a new promoter each...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...where I spent a couple of days talking to researchers about their work, picking up plasmid deposits, and trying...spend a large amount of my time meeting with and talking to graduate students, post-docs, and PIs. I won...enterprise work better and because I really like talking to people about and communicating science. The ...research at BU, I realized that I’m energized by talking to people about science and that I love explaining...running away screaming, but instead can involve walking alongside it and giving it a high five. While screaming...technical writing, teaching, presenting, analytical thinking, time management, information distillation, etc...transferable to many different fields. If you start thinking about preparing yourself for a career while still...

...LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted Dawson 17613...William Hahn 23955 YFP-Parkin PRKN YFP CMV Parkinson's Richard Youle 23956 mCherry-Parkin PRKN mCherry CMV ...2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2-LRRs LRRK2 Myc CMV Parkinson's Mark...His polH Parkinson's Michael J Fox Foundation MJFF 40385 pBACgus4x/Parkin PRKN polH Parkinson's Michael...PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's Mark Cookson...PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson...PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-V5 KD PINK1 V5 CMV Parkinson's Mark Cookson...

...repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...other cytokines, enable communication between cells of the immune system. Interleukins Interleukins are ...factor FLJ40431 Return to top Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...CCL14-CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ...CCL27 chemokine (C-C motif) ligand 27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 CCL28 chemokine (C-C...CX3CL1 chemokine (C-X3-C motif) ligand 1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin...SCYB14, bolekine CXCL16 chemokine (C-X-C motif) ligand 16 CXCLG16, SR-PSOX, SRPSOX CXCL17 chemokine (C-X-...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating protein 35 ARHGEF2...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB... PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...motor neurons leads to limb weakness, difficulty speaking, swallowing, and breathing, and often results ...movements, memory deficits, as well as difficulty speaking and swallowing. HD is caused by abnormal expansion...Symptoms include memory loss, deficits in decision-making, and language abilities. The disease can be classified...A foundation dedicated to finding a cure for Parkinson's disease through an aggressively funded research... of improved therapies for those living with Parkinson's today. The Michael J. Fox Foundation has made...

...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...

...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ...mRFP1 David Liu 140448 QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg...Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim 177180 pDAS12069_...

...cilia (Kogiso et al., 2020) Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body...spike protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the...infectivity (Heurich et al., 2014). PCP4 plasmids - (Purkinje cell protein 4) is involved in beating of airway...body. For a full list of cytokines and related plasmids see our Immunology Resource Guide . Return to top...window) References Andersen, K. G., Rambaut, A., Lipkin, W. I., Holmes, E. C., & Garry, R. F. (2020). The...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living... the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...repression through cytosine methylation with improved kinetics LSD1 — repression through targeted removal of ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...

... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized...Lou, H. J., Turk, B. E., & Rinehart, J. (2018). Kinase substrate profiling using a proteome-wide Serine-Oriented...Rinehart, J., & Harper, J. W. (2015). The PINK1-PARKIN mitochondrial ubiquitylation pathway drives a program...Study Protein Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

...brighter fluorescence and faster kinetics (CaMPARI2) Improved methods for marking active neuron populations....fluorescent calcium sensors (fRCaMP1/2, fRGECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically... novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...Glutamate iGluSnFR glutamate sensor with improved trafficking to the plasma membrane Acute disruption of the...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy... red fluorescent protein to monitor exocytosis docking and fusion steps. Nat Commun. 2021 Mar 3;12(1):...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's disease research. This collection is part of ...

...Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice ...applications. Full references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent... for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224 : sgRNAs...10.1038/nbt.2647. Epub 2013 Jul 21. PubMed . Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing...plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Platt...

...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins....analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...presence of a substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association...