Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 23 results for: 5-Oct

Clear keywords
Showing: 1 - 20 of 23 results

  1. p53 Pathway

    Type
    Collection
    ...3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase...arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah...Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573 . Uncovering the role...p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097 . The expanding...Menendez D, Inga A, Resnick MA. Nat Rev Cancer. 2009 Oct;9(10):724-37. PubMed PMID: 19776742 . Germline TP53...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10):e257. Wolf Frommer Return to top Neurotransmitters...indicator for neural activity imaging. J Neurosci. 2012 Oct 3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren...Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier García-Sancho Calcium Genetically... calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. Amy Palmer , Roger Tsien Calcium Turquoise...marking active neuron populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression... in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent Mg 2+ sensor...Genome Mining Strategies. ACS Sens. 2022 May 27;7(5):1336-1346. Edward Boyden Zinc FRET-based zinc sensor...

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S...Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390/v13102073 Resources ...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... resistance-1 gene (pfmdr1). J Parasitol. 2011 Oct;97(5):939-42. doi: 10.1645/GE-2792.1. Epub 2011 May... quantitative PCR. Expert Rev Mol Diagn. 2005 Mar;5(2):209-19. doi: 10.1586/14737159.5.2.209. PMID: 15833050...-87. doi: 10.1016/j.mimet.2005.09.007. Epub 2005 Oct 10. PMID: 16216354 ...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency....that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting efficiency.....2013.08.021. Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using...2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed . Return to SpCas9 plasmids GeCKO Library...Jan;33(1):102-6. doi: 10.1038/nbt.3055. Epub 2014 Oct 19. PubMed . In vivo genome editing using Staphylococcus... Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014....

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Plasmids and Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID...factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028. PMID: 4878700...concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: 19263463. Ribeiro...multicopy plasmid stability. Mol Microbiol. 1998 Sep;29(5):1137-45. doi: 10.1046/j.1365-2958.1998.01012.x. PMID...

  8. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...their Roller phenotype. If SEC is inserted at the 5’ end of a gene, the SEC separates the targeted gene...are then heat shocked to remove the SEC and after 5-6 days of growth, wild-type animals that have undergone...Ward JD, Reiner DJ, Goldstein B. Nat Methods. 2013 Oct;10(10):1028-34. doi: 10.1038/nmeth.2641. Epub 2013...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...pluripotent stem cells. Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic...fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72. Yamanaka Plasmid Human Non-integrating transient...integration-free human iPS cells. Nat Methods. 2011 May;8(5):409-12. Yamanaka Replicating EBNA1 episome Human ...Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming...Synthetic Modified mRNA. Cell Stem Cell. 2010 Nov 5;7(5):618-30. Rossi RNA Human Non-integrating, polycistronic...Developmental Potential of iPSCs. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. Schöler You can also...hematopoietic progenitor cells. Cell Rep. 2014 Dec 11;9(5):1871-84. Lacaud Fibroblasts Hematopoietic Progenitor...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. (PubMed) Friedrich et al...self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133-9. doi: 10.1021/cb400427b. Epub 2013...

  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated in third generation... size. Opting for a larger vessel, such as a 3 or 5-layer flask will require some initial optimization...limit of lentiviral vectors. Hum Gene Ther. 2001 Oct 10;12(15):1893-905. PubMed PMID: 11589831. Jiang ...preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152. PubMed Central PMCID...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...using the F plasmid ccdB killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard,.... 1993 Dec 5;234(3):534-41. PubMed PMID: 8254658. 5. Bernard, P. and Couturier, M. “Cell killing by the...ccd operon in plasmid F.” J Bacteriol. 1989 May;171(5):2353-60. PubMed PMID: 2651399. PubMed Central PMCID...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transactivator for transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts... is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. Although...PMID: 25075903. PubMed Central PMCID: PMC4486245. 5. Patient monitoring and follow-up in lentiviral clinical...U, Takahashi M, Gage FH, Verma IM. J Virol. 1998 Oct;72(10):8150-7. PubMed PMID: 9733856. PubMed Central...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Edelhoch method. Int J Biol Macromol. 2015;77:260-5. doi: 10.1016/j.ijbiomac.2015.03.027. Epub 2015 Mar...Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi: 10.1016/j.ijpharm.2018.08.043... in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449, ISSN 1359-0294, https://doi....

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...versus analog real-time PCR. Nat Methods. 2013 Oct;10(10):1003-5. PubMed PMID: 22035192. PubMed Central PMCID...PMID: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain...

  17. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Last updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning... best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA locus as assessed by Southern... mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et al., FEBS Lett 2012 Oct 19;586(20):3716-22. Park...transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10):e76117. Irreversible peptide-peptide ligation...creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, up to six...e16765. Engler et al., ACS Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504. For further reading...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Coli Synthetic Biology.” ACS Synthetic Biology, vol. 5, no. 10, American Chemical Society (ACS), 2 May 2016...Traditional Biology.” ACS Synthetic Biology, vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015....” PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p.... vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp. 2593–606. Crossref, https://doi.org... Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016/j.xgen.2024.100519...

  20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...773–779 . https://doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted ...
Showing: 1 - 20 of 23 results