Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 322 results for: INO

Clear keywords
Showing: 1 - 20 of 322 results

  1. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... Protocols Inoculating a Liquid Bacterial Culture Inoculating a Liquid... for Inoculating a Bacterial Culture Protocols...experimental use. The following protocol is for inoculating an overnight culture of liquid LB with bacteria...Watch the protocol video below to learn how to inoculate bacteria in liquid culture. Written Protocol Prepare...is LB media + antibiotic without any bacteria inoculated. You should see no growth in this culture after...µg/mL Gentamycin 10 µg/mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10 µg/mL Tips and FAQ ...

  2. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...mouse and human kinomes. While any subset of the genome is limited by definition, the kinome is a well-annotated... About CRISPR Pooled Library Screening Why the kinome? While properly designed perturbations – in the...incremental approach is prudent. What's in the CRISPR kinome library? Thus, we are glad to provide CRISPR libraries...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs found... kinome...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...Image adapted from Ino et al. 2022, under CC-BY license.   Find MTRIA sensors here!   Ino D, et al. A fluorescent...develop a biosensor for oxytocin, Daisuke Ino and Hiroshi Hibino and colleagues devised a “Modular fluorescence...G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT enabled...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...

  5. Genetic Code Expansion

    Type
    Collection
    ...incorporation of amino acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs). This...protein translation, an aminoacyl-tRNA synthetase (aaRS) enzyme loads a specific amino acid to its tRNA. The...pANAP AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian TAG Peter ...structurally analogous amino acids Bacterial TAG Ryan Mehl 85502 pDule-para-aminoPhe pAF synthetase M. jannaschii...jannaschii para-aminoPhe Bacterial TAG Ryan Mehl 85503 pDule2-para-aminoPhe pAF synthetase M. jannaschii...AnapRS AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-amino-propanoic acid (Anap) Mammalian TAG Simon ...jannaschii 3-aminotyrosine Bacterial TAG Huiwang Ai 153558 pMAH-EcaYRS EcaYRS E. coli 3-aminotyrosine Mammalian...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...ULBP4, bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...PF4A, SCYB4V1 PLAU plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase...KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte growth... DG6, PMBP PLAU plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase...protein 2 FLJ23282, FRA RARA retinoic acid receptor, alpha NR1B1, RAR RARB retinoic acid receptor, beta HAP...NR2B1 RXRB retinoid X receptor, beta DAUDI6, H-2RIIBP, MGC1831, NR2B2, RCoR-1 RXRG retinoid X receptor...

  7. CRISPR Guide

    Type
    Collection
    ...base editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...to small indels in the target DNA that result in amino acid deletions, insertions, or frameshift mutations...enzymes. hfCas9s were developed by mutating specific amino acid residues to reduce off-target editing. Some... prime editing, and are often used to change an amino acid, turn a promoter on/off, or add small protein...with a silent mutation that does not change the amino acid sequence. HDR efficiency is generally low (...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...soon followed, using an enzyme evolved to excise inosine to access the same repair pathway and convert A...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is translated as guanosine, so the overall...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR genes employ varied mechanisms ...MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA...Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. 2017. Multiplex gene...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...1147-1155. Lin Tian Endocannabinoid GRAB_eCB2.0/eCBmut biosensor for endocannabinoids in vivo (Constitutive...CEPIA. Nat Commun. 2014 Jun 13;5:4153. Masamitsu Iino Calcium Red mitochondrial CEPIA calcium sensors ...mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red calcium sensor FRCaMPi and SomaFRCaMPi...with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent Protein and PDE5alpha...sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo. Nat Biotechnol. 2021 Nov ... J Neurosci. 2006 Feb 15. 26(7):2000-9. Robert Malinow Endo/Exocytosis Synaptophysin-pHluorin Clathrin-mediated...

  10. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ... Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell ....top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  12. Bacterial Expression Systems

    Type
    Collection
    ...Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC...smegmatis Matthias Wilmanns 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias...Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias... bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus) Escherichia ...Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry...

  13. Ras Pathway

    Type
    Collection
    ...Platelet derived growth factor receptor PDPK1 3-phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine...PIK3R4 PIK3R5 PIK3R6 Also known as PI3K; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...phosphatase 1 catalytic subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...

  15. Brain Initiative Collection

    Type
    Collection
    ...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  16. mTOR Pathway

    Type
    Collection
    ...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth...PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ...

  17. Microbiology Resources

    Type
    Collection
    ...Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. ...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...

  18. TALEN Guide

    Type
    Collection
    ...composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their ...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag...
Showing: 1 - 20 of 322 results