We narrowed to 325 results for: sars

...this novel virus. SARS-CoV-2 entry into a host cell, like that of SARS-CoV and MERS CoV, ...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is the angiotensin receptor, Angiotensin...antibody targeting the SARS-CoV-2 nucleocapsid protein. Brian Geiss 211757 Anti-SARS-CoV-2 Nucleocapsid Protein...antibody targeting the SARS-CoV-2 nucleocapsid protein. Brian Geiss Plasmids SARS-CoV-2 Plasmids Browse...Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV, MERS,...TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find plasmids related to these genes...Host cell receptor mediating the entry of SARS-CoV and SARS-CoV-2 viruses (Anderson et al., 2020). TMPRSS2...

...Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV-2 infection...Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids...pseudotyping with SARS-CoV-2 spike protein. Collections... Collections COVID-19 – Viral Pseudotyping SARS-CoV-2 Pseudotyped...viral spread. As a Biosafety Level 3 (BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study...as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface...cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of viral entry and infection...

...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems....experiments. In an effort to speed up research of SARS-CoV-2 in early 2020, Ginkgo Bioworks (Link opens...build DNA that would enable researchers to express SARS-CoV-2 viral proteins in several expression systems...Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on: Gene...Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems Collection...

... for SARS-CoV-2 spike pseudotyping, visit the SARS-CoV-2 Pseudotyped Virus collection page. SARS-CoV-2...reaction to detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun ...functional receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and ...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from ...labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein to bind...and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids...

...developed during the SARS-CoV pandemic more than a decade ago are now being used to study SARS-CoV-2. The resurgence...replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for clinical manifestations... glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun 11... need fast access to the tools required to study SARS-CoV-2, but with many academic research labs closed... as biobanks, are crucial for the advancement of SARS-CoV-2 research because they provide rapid access...Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are now important...susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional cell line...

...pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2...the SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-...lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins...protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro...last few months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs...

... 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the IGI.   What SARS-CoV-2 plasmids from...Ginkgo Bioworks to synthesize and share over 1,850 SARS-CoV-2 plasmids to help academic and industrial labs... for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...bacterial expression systems, Ginkgo synthesized SARS-CoV-2 expression plasmids in a high copy backbone...for the high level expression and purification of SARS-CoV-2 proteins for downstream experiments and offer...optimized, full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven...Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae...

... coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants...several months, we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination...patients. They were able to tweak this assay to study SARS-CoV-2 spike variants. “We created a series of spike...vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization assay. Neutralization...on the effectiveness of current vaccines against SARS-CoV-2 variants, this study was the first to compare...Lessells RJ, de Oliveira T, Sigal A (2021) Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent...Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-...

...from Iowa State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity... by primers that target the E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the ... similar method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA...single-molecule sensitive. In the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification...easy-to-deploy, protocol for Cas13-based detection of SARS-CoV-2 genetic material (CREST) Sensitivity: 10 copies...

...comprehensive SARS-CoV-2 virus-human interaction network in which we expressed 26 of the 29 SARS-CoV-2 viral...since we all became acutely aware of the threat of SARS-CoV-2. Like many in the science community, we’ve...lifecycle, identifying these potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is...We are just at the beginning of understanding how SARS-CoV-2 interacts with human proteins. For example...scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various ways...key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 and Nsp16, ...out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing...

...Addgene repository of tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein...mBG17] and Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid.... Find Anti-SARS-CoV-2 Antibodies here! Terry, J.S., et al. (2021). Development of a SARS-CoV-2 nucleocapsid...Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2...07259-6. PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use ...    Figure 3: HeLa cells transduced with SARS-CoV-2 nucleocapsid protein (Plasmid #141391-LV [...negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left...

...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday...Sunday, June 9th, we hosted our 20th anniversary party for all Addgenies to celebrate together. We were even...

...post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA ...-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape... the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing....display library. From Starr et al Science., 2021. SARS-CoV-2 infects host cells through binding the human...responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes...protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s...

... Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped...this lovely play-by-play of Addgene's 10 Year Anniversary Gala. The Addgene lab often works as a very well-oiled... addgene's 10 year anniversary...

...reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We also highlighted...plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope that by providing...working with RNA and extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR...detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited at...

...NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins...

...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into ...role in infecting cell lines without using intact SARS-CoV-2 virus. Scientists have been using similar ...2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike...Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays. Viruses...

...also offer export license applications for VSV and SARS-COV-1 plasmids. Export control license process  ... is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since...

...pandemic, qPCR can be used to diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and...has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The test is started using RNA purified... recently adapted the DETECTR platform to detect SARS-CoV-2 using a lateral flow strip format. They published..., Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038...

...rapidly identify and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound...Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) ...et al. (2020) Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https:/...