Skip to main content
Addgene
Showing: 361 - 400 of 671 results

  1. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Antibodies: Historical Perspective and Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589... Ashley Waldron...

  3. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...splice acceptors. They require insertion into an intron of a gene, where they can be spliced into the gene...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...It is probably good news for our environment that more people are paying attention to living a “green...reagents and buffers.  However, I had very environmentally conscious friends who had me thinking about...

  6. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...interests and areas of expertise. Perhaps the strongest aspect of science communication, then, is its ...allowed to percolate within a new intellectual environment. Over time, this interest came to incorporate...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...with ever more efficient tools for light mediated neuronal control and we are expanding our viral service...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...

  11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

  13. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  14. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently join...-executed cloning endeavor, NEBuilder requires upfront planning. This cloning methodology requires that...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...shipped), where they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours...PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M,...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...10.5%  3.2  534.1 3 2010 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010... 2010 167  Applied and Environmental Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...CRISPRainbow. Both tools were targeted to the FBN3 intronic repeat which contains 22 copies of the gRNA target...distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric region. All pairs...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found.... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...

  20. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...generating knock-in mutations, and NHEJ is an error-prone pathway frequently introducing frameshift knockout...Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389/fmicb.2020.607028...

  21. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...disease mutation in basal forebrain cholinergic neurons derived from iPSCs. From Rohn et al. 2018; see ... Limitations, and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 ...

  22. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...high GC content and hairpin structures, ITRs are prone to replication errors and disruptive mutations and...improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085...

  24. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...number, but it was attained without any of the error-prone repair that normally accompanies Cas9 cutting. We... holds some kind of fitness advantage but error-prone repair of the gene would be disastrous (i.e. if ...

  25. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...effective delegation skills: The team will be stronger and less dependent on the manager to succeed. ... your team and take responsibility if things go wrong. Here are some important criteria for delegating...

  26. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool...genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  30. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...immunogenicity, which means they do not stimulate a strong immune response when introduced into an organism...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors...

  31. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... use amplicons in ITR as we have found this to strongly bias titer. We are also adopting ddPCR, which ...microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and...

  32. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann ...doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

  33. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...make a knockout in your gene of interest, as it is prone to generating indel errors. Indel errors generated...Non-Homologous End-Joining Really an Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org...

  34. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...

  36. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence...TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system for ...

  37. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ... psoriasis Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes...

  38. RNA Extraction Without A Kit

    Type
    Blog Post
    ...degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be required- something...late 1970’s, Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did...

  39. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ... Ashley Waldron...

  40. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...particularly helpful when wanting to target specific neuron subtypes, since AAVs tend to be very neuro-permissive...Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral tools.   References...
Showing: 361 - 400 of 671 results